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Zymo DNA Clean & Concentrator-500
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- Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
- Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Product Description
The DNA Clean & Concentrator-500 (DCC-500) is our highest capacity PCR purification kit of the DNA Clean & Concentrator products. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.
Technical Specifications
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 2 ml |
| Equipment | Microcentrifuge and centrifuge or vacuum source. |
| Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Sample Source | DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 500 µg total DNA can be recovered. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
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