- Home
- Zymo Research Kits
- ZymoPURE - Express Plasmid Midiprep Kit, 25 preps
ZymoPURE - Express Plasmid Midiprep Kit, 25 preps
- Details
- Send to friend
- Customer feedback
- Direct lysis protocol omits conventional cell-pelleting and resuspension steps.
- Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
- Prepare transfection-grade plasmid DNA in only 15 minutes.
Product Description
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE – Express Plasmid Midiprep kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of transfection-grade plasmid DNA directly from E. coli culture without conventional cell-pelleting and resuspension steps. The ZP Express Lysis Buffer is simply added directly to the culture and the plasmid DNA is purified following neutralization and removal of debris using the patented ZymoPURE binding system.
The plasmid DNA is rapidly bound onto a column with a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The ZymoPURE – Express Plasmid Midiprep kit’s fast and simple protocol enables the recovery of highly concentrated transfection-grade plasmid DNA in just 15 minutes. As an added convenience, the ZymoPURE – Express Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
|---|---|
| Binding Capacity | 1.2 mg |
| Culture Input | 25 ml |
| Elution Volume | ≥ 150 µl |
| Endotoxin Levels | ≤1 EU/µg of Plasmid DNA |
| Equipment | Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge |
| Format | Spin-Column |
| Processing Time | 15 min |
| Purity | Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0 |
| Recommended Sample Volume | ≤ 25 ml |
| Size Range | Up to 200 kb |
| Yield | Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 25 ml of overnight culture grown in LB for high copy number plasmids are 100 – 200 µg. |
