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- ZymoPURE II Plasmid Gigaprep Kit, 5 preps
ZymoPURE II Plasmid Gigaprep Kit, 5 preps
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- Perform plasmid gigapreps in only 45 minutes using a simple spin-column protocol.
- Purify up to 25 mg of highly concentrated plasmid DNA directly from a spin-column.
- Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.
Product Description
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 25 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Gigaprep kit can process up to 2.5 L of culture in less than 45 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with a vacuum instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Gigaprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Technical Specifications
| Applicable For | Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications. |
|---|---|
| Binding Capacity | 25 mg |
| Culture Input | ≤ 2.5 L |
| Elution Volume | ≥ 3.0 ml |
| Endotoxin Levels | ≤ 0.025 EU/µg of Plasmid DNA |
| Equipment | Vacuum/vacuum manifold and swinging bucket centrifuge |
| Format | Spin-Column |
| Processing Time | ≤ 45 min |
| Purity | Typical Abs 260/280 ≥1.8 and Abs 260/230 ≥ 2.0 |
| Recommended Sample Volume | ≤ 2.5 L |
| Size Range | Up to 200 kb |
| Yield | Up to 3.0 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 150 ml of overnight culture grown in LB for high copy number plasmids are 600 – 1,200 µg. |
