Product Description
LIGHT LABS is proud to offer the CELLTREAT 300cm² Tissue Culture Treated Flask with Vent Cap (Cat # 229361) — the largest standard tissue culture flask in CELLTREAT's comprehensive TC-treated flask line, providing 300cm² of growth area and a 1250mL maximum volume for maximum-scale adherent mammalian cell culture. The 229361 is the go-to flask when the highest possible cell yield per vessel is needed from a standard flask format — ideal for large-scale protein expression, viral vector production, cell banking, and any workflow requiring the largest available single-layer adherent culture surface in a standard flask footprint.
The tissue culture treated surface delivers a consistent, uniform hydrophilic surface — manufactured as a negatively charged hydrophilic surface using USP Class VI virgin polystyrene in a class 100,000 cleanroom — resulting in flat, striation-free growth surfaces that maximize usable growth area and optical transparency across the full 300cm² footprint. The molded interlocking T-design on the flask base enables secure, space-efficient stacking with other compatible CELLTREAT flask sizes (75, 182, and 300cm², and 250, 600, and 850mL formats). The large opening canted neck design with moderate angles provides easy access to the entire flask surface for pipettes and cell scrapers — particularly important for complete cell harvest from a large-format flask. The green HDPE vent cap with 0.22µm hydrophobic membrane maintains sterility while allowing consistent gas exchange with the CO₂ incubator atmosphere. Every flask is printed with lot number and expiration date for quality traceability and is packaged in convenient zip-closure resealable bags. LIGHT LABS is your trusted source for CELLTREAT laboratory plasticware.
KEY FEATURES
- 300cm² TC-treated growth area — largest standard TC-treated flask format in the CELLTREAT line; maximum cell yield per single-layer adherent vessel
- 1250mL maximum volume — accommodates the full range of media volumes for large-scale culture in a standard flask format
- Tissue culture treated (TC-treated) surface — negatively charged uniform hydrophilic surface; flat, striation-free growth area for maximum usable culture surface and optical transparency
- Manufactured under class 100,000 cleanroom conditions using USP Class VI virgin polystyrene
- 100% flask integrity tested during manufacturing for leak-free reliability
- Vent cap with 0.22µm hydrophobic membrane — allows CO₂/gas exchange with incubator atmosphere while maintaining sterility; green HDPE cap
- Molded interlocking T-design — compatible secure stacking with CELLTREAT 75, 182, and 300cm² flasks and 250, 600, and 850mL flasks
- Large opening, canted neck design with moderate angles — easy access to entire flask surface with pipettes and cell scrapers for complete cell harvest
- Stable flat base — flask stands upright during filling without a stand or holder
- Thick, durable polystyrene walls for superior structural integrity
- Every flask printed with lot number and expiration date for quality traceability
- Convenient zip-closure resealable bag packaging
- Sterile — gamma irradiated
- Certified nonpyrogenic and RNase-/DNase-free
- Packaged 3 flasks per resealable bag; 6 bags per case (18 flasks/case)
- Fisher Scientific Catalog No. 50-403-871
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | 229361 |
| Fisher Scientific Catalog No. | 50-403-871 |
| Brand | CELLTREAT Scientific Products |
| Product Name | 300cm² TC-Treated Tissue Culture Flask with Vent Cap |
| Growth Area | 300 cm² |
| Maximum Volume | 1250 mL |
| Surface Treatment | Tissue Culture Treated (TC-Treated) |
| Cap Type | Vent Cap (0.22µm hydrophobic membrane) |
| Cap Color | Green HDPE |
| Neck Design | Canted neck, large opening, moderate angle |
| Base | Stable flat base (stands upright) |
| Stacking Feature | Molded interlocking T-design |
| Material | USP Class VI virgin polystyrene (clear); Green HDPE (cap) |
| Manufacturing Environment | Class 100,000 cleanroom |
| Integrity Testing | 100% tested |
| Sterility | Sterile (gamma irradiated) |
| Certifications | Nonpyrogenic, RNase-free, DNase-free |
| Lot/Expiry Printing | Yes — lot number and expiration date printed on each flask |
| Packaging | 3 flasks/resealable zip-closure bag; 6 bags/case |
| Quantity | 18 flasks/case |
APPLICATIONS
- Maximum-scale mammalian cell line maintenance and passaging in a standard flask format
- Large-scale protein expression in adherent cell systems
- Viral vector (lentiviral, AAV, adenoviral) production requiring maximum cell yield per vessel
- Cell banking and cryopreservation requiring the highest possible cell numbers per flask
- Large-scale transfection and stable cell line generation
- Bulk cell harvesting for downstream processing
- Scale-up experiments at the maximum single-layer flask scale before transitioning to multi-layer formats
- Any adherent cell culture application requiring the largest standard TC-treated flask with vent cap
