Product Description
- Streamlined, proven procedure for bisulfite conversion of DNA.
- Desulphonation and recovery of bisulfite-treated DNA with a spin column.
- Recovered DNA is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applications | Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc. This kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array. |
|---|---|
| Conversion | >99% |
| Elution Volume | ≥10 µl |
| Equipment | Microcentrifuge and thermocycler with heated lid |
| Input | 500 pg - 2 µg of DNA |
| Processing Time | 12-16 hours |
| Recovery | >80% |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| Supplemental Info |