Product Description
- Automation-specific streamlined design for high-throughput bisulfite conversion of DNA for methylation analysis.
- Compatible with Illumina® Infinium™ MethylationEPIC, HTS iSelect® Methyl Custom, and Mouse Methylation arrays.
- Optimized buffer volumes for consistent automation performance.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Binding Capacity | 1 µg total DNA per 5 µl MagBinding Beads |
|---|---|
| Conversion Efficiency | > 99.5% of non-methylated C residues are converted to U; > 99.5% protection of methylated cytosines. |
| DNA Purity | A260/A280 & A260/A230 ≥ 1.8 |
| Input | 100 pg - 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. |
| Input Recovery | > 80% (≥ 100 bp) |
| Required Equipment | Thermal cycler with heated lid. |
| Sample Source | Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free. |
| User Provided Materials | Ethanol (95 – 100%), non-skirted 96-well microplates, and 96-well microplate sealing films. See Recommendations for instrument hardware and labware requirements. |
| Supplemental Info |
