Product Description
- Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
- Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
- High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applicable For | Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 60 µl TE or DNase-free Water |
| Equipment | Microcentrifuge, heat block/bath |
| Processing Time | 1.5 hours |
| Processing Volume | ≤ 1.5 ml of Culture (1-5 x 107 cells) |
| Purity | Typical A260/A280 & A260/A230 ≥ 1.8 |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA. |
| Type | Total DNA |
| Yield | ≤ 25 µg of total DNA |