Product Description
- High Purity: Purify endotoxin-free BAC/PAC plasmid DNA (up to ~200 kb) for sequencing, PCR, restriction endonuclease digestion, etc.
- Colored Buffers: P1, P2, and P3 buffers are colored for easy visualization of complete lysis and neutralization
- Unique column design: Zero buffer retention and low elution volume (10 µl)
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Applicable For | Transfection, transformation, sequencing, restriction endonuclease digestion, PCR, in vitro transcription, and other sensitive applications. |
|---|---|
| Elution Volume | ≥ 10 µl |
| Equipment | Microcentrifuge |
| Processing Time | 15 min |
| Purity | Typical Abs 260/280 ≥ 1.8 and Abs 260/230 ≥ 2.0 |
| Size Range | Up to ~200 kb |
| Yield | Up to 10 µg per preparation, depending on the plasmid copy number, initial volume of E. Coli culture processed, and culture growth conditions. The typical yield is 1 µg per 3 ml of culture. |
