Product Description
- Enrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing.
- Inactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA/RNA Shield.
- Remove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS.
DESCRIPTION
TECHNICAL SPECIFICATIONS
| Equipment Needed | Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter/pulverizer (optional) |
|---|---|
| Format | Bead beating, spin column. |
| RNA Purity | High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage. |
| Sample Inactivation | DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures. |
| Sample Input | Water, wastewater, sludge, sewage, biofilms, etc. |
| Sample Size | Up to 5 mL |
| Yield | Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample. |
