Zymo Research D4201 ZymoPURE™ II Plasmid Midiprep Kit — 50 Preps

Product Description

The Zymo Research D4201 ZymoPURE™ II Plasmid Midiprep Kit is the fastest and highest-yielding midiprep kit available — purifying up to 400 µg of transfection-grade plasmid DNA from E. coli in less than 20 minutes, with no ethanol precipitation step and no gravity flow column. Using a modified alkaline lysis system combined with Zymo Research's novel binding chemistry, the ZymoPURE II Midiprep delivers highly concentrated, EndoZero™ plasmid DNA (up to 3 µg/µL) directly from a spin column — ready for immediate use in even the most demanding downstream applications. The D4201 is the 50-prep format, providing the best per-prep value in the ZymoPURE II Midiprep line.

Fastest Midiprep Available — Complete in Under 20 Minutes Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Midiprep kit can process up to 50 mL of culture in less than 20 minutes. There is no ethanol precipitation step — the elution is performed directly in a microcentrifuge — dramatically reducing hands-on time compared to conventional gravity-flow midiprep columns. For labs that routinely run midipreps for transfection, in vivo studies, or scale-up cloning work, the time savings are significant.

Up to 400 µg Yield — Directly from a Spin Column The ZymoPURE II Midiprep routinely delivers up to 400 µg of highly concentrated plasmid DNA (up to 3 µg/µL) in a small elution volume — directly from the Zymo-Spin™ column without requiring precipitation or concentration steps. The eluted DNA is immediately ready for transfection, sequencing, restriction digestion, or long-term storage.

EndoZero™ — Vaccine-Grade Endotoxin Removal Coupling ZymoPURE with the innovative EndoZero™ II Spin-Columns eliminates endotoxins, achieving EndoZero plasmid DNA (≤0.025 EU/µg of plasmid DNA) — vaccine-grade purity suitable for transfection of even the most sensitive cell lines, in vivo studies, and other applications where endotoxin levels are strictly controlled. This level of endotoxin removal exceeds what standard midiprep kits provide, making the ZymoPURE II the clear choice for labs working with primary cells, in vivo delivery, or regulatory-grade material.

Vacuum or Centrifuge Compatible The unique Zymo-Spin™ column design allows the binding step to be performed using either a vacuum manifold or a standard tabletop centrifuge — providing flexibility for labs with different equipment setups or throughput requirements.

Color-Coded Buffers for Error-Free Processing The ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. P1 is Red, P2 is Blue, and P3 is Yellow — providing unambiguous visual confirmation at every critical step of the alkaline lysis protocol, eliminating one of the most common sources of failed midipreps.

Syringe Filters Included — No Centrifuge Clearing Required Syringe filters are included in the kit for rapid, simple clearing of the bacterial lysate — eliminating the need for a high-speed centrifugation step to pellet the cell debris. This further reduces the time and equipment required to complete each prep.

Kit Components (50 Preps — D4201)

• ZymoPURE™ P1 (Red) — contains RNase A (100 µg/mL); store at 4–8°C for long-term storage
• ZymoPURE™ P2 (Blue)
• ZymoPURE™ P3 (Yellow)
• ZymoPURE™ Binding Buffer
• ZymoPURE™ Wash 1
• ZymoPURE™ Wash 2 (Concentrate)
• ZymoPURE™ Elution Buffer
• Zymo-Spin™ V-PS Column Assemblies with 15 mL Reservoir-X (50)
• ZymoPURE™ Syringe Filter-X (50)
• Syringe Plungers (50)
• Collection Tubes (50)
• Instruction Manual

ZymoPURE II Midiprep vs. Miniprep vs. Maxiprep — Choosing the Right Format

Available Pack Sizes — ZymoPURE II Midiprep:

• D4200 — ZymoPURE II Plasmid Midiprep Kit, 25 Preps
• D4201 — ZymoPURE II Plasmid Midiprep Kit, 50 Preps (this product)

Key Features

• Up to 400 µg yield per prep directly from spin column
• Up to 3 µg/µL DNA concentration — no precipitation needed
• Complete protocol in ≤20 minutes from culture to eluted DNA
• EndoZero™ — ≤0.025 EU/µg endotoxin (vaccine-grade)
• No ethanol precipitation step
• No gravity flow column
• Vacuum or centrifuge compatible
• Syringe filters included for fast lysate clearing
• Color-coded buffers: P1 Red, P2 Blue, P3 Yellow
• Supports up to 50 mL overnight E. coli culture
• Low-copy number protocol included
• Gram-positive bacteria protocol included
• 50 preps per kit
• 1-year kit integrity guarantee; lot-to-lot tested
• All steps at room temperature (15–30°C)

Supported Applications Cell transfection, in vivo delivery, viral vector production support, bacterial transformation, restriction endonuclease digestion, DNA sequencing, PCR amplification, plasmid-mediated gene silencing, cloning, and any application requiring large quantities of ultra-pure, endotoxin-free plasmid DNA.

Technical Specifications