Zymo Research D4202 ZymoPURE II™ Plasmid Maxiprep Kit — 10 Preps

The Zymo Research D4202 ZymoPURE II™ Plasmid Maxiprep Kit is the fastest and simplest plasmid maxiprep kit available, delivering up to 3 mg of transfection-grade, endotoxin-free plasmid DNA from up to 150 mL of E. coli culture in under 18 minutes. The D4202 is the 10-prep version of the ZymoPURE II — ideal for labs running occasional maxipreps or evaluating the kit before committing to a larger quantity. Designed for demanding applications including transfection, CRISPR, lentiviral vector production, and in vivo studies, the ZymoPURE II sets a new standard for speed, yield, and DNA purity at the maxiprep scale.

Patented Binding System — Faster, Higher Yields Unlike traditional gravity-flow maxiprep columns, the ZymoPURE II uses a patented binding chemistry in combination with modified alkaline lysis to dramatically accelerate processing. Plasmid DNA binds rapidly to the Zymo-Spin™ V-PX Column using either vacuum or centrifugation — eliminating the slow gravity flow step that makes conventional maxipreps so time-consuming. There is no alcohol precipitation step required, and elution is performed directly into a microcentrifuge tube for immediate use.

Highly Concentrated, Transfection-Ready DNA The ZymoPURE II delivers highly concentrated plasmid DNA — up to 6 µg/µL directly from the spin column — with typical purity of A260/280 ≥ 1.8 and A260/230 ≥ 2.0. Endotoxin levels are ≤ 0.025 EU/µg, making the eluted DNA suitable for the most sensitive downstream applications including primary cell transfection, stem cell work, lentivirus and AAV production, and in vivo delivery.

Color-Coded Buffers for Error-Free Processing The ZymoPURE II Plasmid Maxiprep kit contains color-coded buffers (Red, Blue, Yellow) that allow error-free visualization and identification of complete bacterial cell lysis and neutralization — a practical safeguard that eliminates a common source of failed preps, particularly valuable for less experienced users or high-throughput environments.

Kit Components (10 Preps)

• ZymoPURE™ P1 — Red (1 × 150 mL)
• ZymoPURE™ P2 — Blue (1 × 150 mL)
• ZymoPURE™ P3 — Yellow (1 × 150 mL)
• ZymoPURE™ Binding Buffer (1 × 150 mL)
• ZymoPURE™ Wash 1 (1 × 55 mL)
• ZymoPURE™ Wash 2 Concentrate (1 × 23 mL)
• ZymoPURE™ Elution Buffer (6 mL)
• Zymo-Spin™ V-PX Column Assemblies (10)
• ZymoPURE™ Syringe Filters (10)
• ZymoPURE™ Syringe Plungers (10)
• Collection Tubes (10)
• Instruction Manual

Supported Applications Transfection, lentivirus production, adenovirus production, AAV production, CRISPR and genome editing, in vivo studies, restriction endonuclease digestion, in vitro transcription/translation, DNA sequencing, PCR, bacterial transformation, and other sensitive downstream applications.

Also Available:

• D4203 — ZymoPURE II Plasmid Maxiprep Kit, 20 Preps
• D4211 — ZymoPURE Plasmid Miniprep Kit, 400 Preps
• D4212 — ZymoPURE Plasmid Miniprep Kit, 800 Preps
• D4027 — Zyppy Plasmid Maxiprep Kit, 10 Preps
• D4028 — Zyppy Plasmid Maxiprep Kit, 20 Preps

Key Features

• Up to 3 mg plasmid DNA per prep (up to 6 µg/µL concentration)
• ≤ 18 minute processing time from culture to elution
• Processes up to 150 mL of E. coli culture
• No alcohol precipitation step required
• Vacuum or centrifuge compatible binding step
• Endotoxin-free (≤ 0.025 EU/µg DNA)
• Transfection-ready, vaccine-grade quality
• Color-coded buffers for error-free lysis verification
• Compatible with plasmids up to 200 kb
• Storage temperature: Room temperature

Technical Specifications