Zymo Research D4210 ZymoPURE™ Plasmid Miniprep Kit — 100 Preps

The Zymo Research D4210 ZymoPURE™ Plasmid Miniprep Kit is a high-performance spin column-based miniprep kit that delivers up to 100 µg of ultra-pure, endotoxin-free, transfection-grade plasmid DNA from up to 5 mL of E. coli culture in less than 15 minutes. With 50,000 times fewer endotoxins than industry-leading miniprep kits and a minimum elution volume of just 25 µL, the ZymoPURE Miniprep sets a new benchmark for miniprep purity and concentration. The D4210 is the 100-prep format — ideal for labs that run minipreps periodically, want to trial the kit before scaling up, or need a smaller stock for lower-throughput workflows.

Transfection-Grade Purity at the Miniprep Scale The ZymoPURE Miniprep's standout feature is its endotoxin performance: ≤ 1 EU/µg — 50,000 times fewer endotoxins than conventional miniprep kits. This makes ZymoPURE miniprep DNA suitable for direct use in transfection, lentiviral vector production, AAV production, and in vivo studies without the additional endotoxin removal steps typically required when using standard miniprep kits. The wash regimen is specifically optimized to eliminate endotoxins, salt, protein, and RNA from the final eluate.

Novel Binding Chemistry — Highest Miniprep Yield ZymoPURE technology uses a modified alkaline lysis method paired with a novel binding chemistry that yields highly concentrated plasmid DNA — up to 3 µg/µL — in an elution volume as low as 25 µL. The unique spin-column design provides zero buffer retention, ensuring maximum recovery in minimum volume. This level of concentration is typically only achievable with precipitation steps that the ZymoPURE eliminates entirely.

Fast — Under 15 Minutes From culture to ready-to-use plasmid DNA in less than 15 minutes. Compatible with both microcentrifuge and vacuum manifold setups for flexible workflow integration.

BAC / YAC / PAC Compatible Unlike most miniprep kits limited to standard plasmids, the ZymoPURE Miniprep handles large constructs up to approximately 200 kb — including BACs, YACs, and PACs — making it a versatile choice for synthetic biology, genomics, and specialized cloning work.

Color-Coded Buffers for Error-Free Processing ZymoPURE's colored buffer system (Red, Blue, Yellow) allows visual confirmation of complete bacterial cell lysis and neutralization at each step — a practical safeguard against failed preps that is especially valuable in high-throughput or teaching lab environments.

Available in Multiple Pack Sizes:

• D4210 — ZymoPURE Plasmid Miniprep Kit, 100 Preps (this product)
• D4211 — ZymoPURE Plasmid Miniprep Kit, 400 Preps
• D4212 — ZymoPURE Plasmid Miniprep Kit, 800 Preps

Also Available — ZymoPURE Maxiprep Kits:

• D4202 — ZymoPURE II Plasmid Maxiprep Kit, 10 Preps
• D4203 — ZymoPURE II Plasmid Maxiprep Kit, 20 Preps

Key Features

• Up to 100 µg plasmid DNA per prep
• Minimum elution volume: 25 µL (up to 3 µg/µL concentration)
• ≤ 15 minute processing time
• 50,000× fewer endotoxins than industry-leading miniprep kits
• Endotoxin level: ≤ 1 EU/µg
• Modified alkaline lysis with novel binding chemistry
• Zero buffer retention spin-column design
• Color-coded buffers for error-free lysis verification
• BAC/YAC/PAC compatible — up to ~200 kb
• Compatible with microcentrifuge and vacuum manifold
• 100 preps per kit — ideal for low-to-medium throughput labs
• Storage temperature: Room temperature

Supported Applications Transfection, lentivirus production, adenovirus production, AAV production, CRISPR and genome editing, in vivo studies, DNA sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, bacterial transformation, and other sensitive downstream applications.

Technical Specifications