Product Description
Keep your yeast plasmid preps running without interruption by restocking the lysis step on its own. This Zymo Research alkaline lysis buffer releases plasmid DNA from Zymolyase-digested yeast cells, matching the original formulation used in the Zymoprep Yeast Plasmid Miniprep I so results stay consistent from batch to batch.
This listing is for catalog number D2001-2-15: Solution 2 Lysis Buffer, 15 mL. After the cell wall is digested in Solution 1, this buffer is added to lyse the spheroplasts and denature proteins and genomic DNA under alkaline conditions. Neutralization with Solution 3 then precipitates debris, leaving the plasmid in solution for recovery. The 15 ml bottle aligns with the Miniprep I kit format. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Alkaline lysis – Releases plasmid DNA from Zymolyase-treated yeast cells.
- Workflow matched – Corresponds to Solution 2 of the Zymoprep Yeast Plasmid Miniprep I.
- Consistent results – Same chemistry as the kit component for reproducible preps.
- Convenient size – Supplied as a 15 ml bottle.
Specifications
| Catalog Number | D2001-2-15 |
| Brand | Zymo Research |
| Description | Solution 2 Lysis Buffer, 15 mL |
| Volume | 15 ml |
| Use | Alkaline lysis of Zymolyase-digested yeast |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- Yeast plasmid miniprep
- Plasmid rescue from fungi
- Alkaline lysis of spheroplasts
