Product Description
Tough yeast and fungal cell walls give way more easily with the right digestion conditions. This Zymo Research buffer provides the environment Zymolyase needs to break down those walls, preparing cells for efficient lysis and plasmid or DNA recovery. It is a convenient way to restock the first step of your yeast plasmid workflow without ordering a complete kit.
This listing is for catalog number D2004-1-10: Solution 1 Digestion Buffer, 10 mL. Used as the first solution in the Zymoprep yeast plasmid procedures, the buffer is combined with Zymolyase and the cell suspension, then incubated so the enzyme can digest the wall before alkaline lysis. Several bottle sizes are offered, from 10 ml up to 90 ml, so you can match supply to your prep volume and throughput. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Enzyme-optimized – Formulated for use with Zymolyase to digest yeast and fungal cell walls.
- Kit replacement – Restocks the first solution of Zymoprep yeast plasmid workflows.
- Multiple sizes – Available in 10, 15, 45 and 90 ml volumes.
- Gentle preparation – Enzymatic digestion avoids beads and harsh mechanical disruption.
Specifications
| Catalog Number | D2004-1-10 |
| Brand | Zymo Research |
| Description | Solution 1 Digestion Buffer, 10 mL |
| Volumes | 10 ml, 15 ml, 45 ml, 90 ml |
| Use | With Zymolyase for yeast and fungal cell wall digestion |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
- D2001-1-15 – Solution 1 Digestion Buffer, 15 mL
- D2004-1-45 – Solution 1 Digestion Buffer, 45 mL
- D2004-1-90 – Solution 1 Digestion Buffer, 90 mL
Applications
- Yeast plasmid miniprep
- Fungal cell wall digestion
- Spheroplast preparation
- Fungal DNA purification
