Product Description
Get microbiome data that reflects the community you actually sampled. This kit is built to lyse tough and fragile organisms with equal efficiency, so Gram-positive bacteria, fungi and protozoa are not under-represented next to easy-to-lyse species. It is a dependable starting point for soil, stool and other complex samples.
This listing is for catalog number D4300: ZymoBIOMICS DNA Miniprep Kit, 50 Preps. Samples are mechanically disrupted by bead beating with ultra-high-density BashingBeads, then DNA is purified on a spin column and passed through OneStep PCR Inhibitor Removal to clear humic acids and other inhibitors common in environmental material. Recovered DNA is typically 15 to 20 kb and ready for PCR, arrays, 16S rRNA gene sequencing and shotgun metagenomics. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Unbiased lysis – Uniform disruption of Gram-negative and Gram-positive bacteria, fungi, protozoa and algae.
- BashingBead technology – Ultra-high-density beads for thorough mechanical lysis.
- Inhibitor removal – OneStep PCR Inhibitor Removal enables PCR from difficult samples.
- Sequencing ready – DNA suited to 16S and shotgun metagenomic workflows.
- Broad sample range – Handles a variety of microbial and environmental inputs.
Specifications
| Catalog Number | D4300 |
| Brand | Zymo Research |
| Description | ZymoBIOMICS DNA Miniprep Kit, 50 Preps |
| DNA size | 15–20 kb |
| Lysis method | Bead beating with BashingBeads |
| Inhibitor removal | OneStep PCR Inhibitor Removal |
| Format | Spin column |
| Pack sizes | 5 or 50 preps |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
| Note | [D4304] ZymoBIOMICS Miniprep Kit x 1, [S6012-50] ZR BashingBead Lysis Tubes 50 pack x 1 [D4300-1-40] ZymoBIOMICS Lysis Solution, 40 ml x 1 |
Available Sizes & Components
Applications
- 16S rRNA gene sequencing
- Shotgun metagenomic sequencing
- Microbial community profiling
- PCR and qPCR
- Microarrays
