Product Description
Direct bisulfite conversion begins with breaking down proteins in the sample, and this buffer sets the conditions for that first step. A replacement supply lets labs extend their EZ DNA Methylation-Direct kits or run additional blood, tissue, and cell samples without reordering the entire kit.
This listing is for catalog number D5021-9: M-Digestion Buffer (2X), 15 mL. Supplied at 2X concentration, the buffer is combined with sample and proteinase K for digestion before the conversion reagent is added. Follow the kit protocol for mixing ratios and incubation conditions. It is the same component included in the EZ DNA Methylation-Direct kits and comes in 4 ml and 15 ml sizes. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Proteinase K digestion – Provides reaction conditions for digesting samples before conversion.
- 2X concentrate – Mixed with sample to reach working strength.
- Kit-matched component – Identical to the buffer in EZ DNA Methylation-Direct kits.
- Two volumes – Offered in 4 ml and 15 ml sizes.
Specifications
| Catalog Number | D5021-9 |
| Brand | Zymo Research |
| Description | M-Digestion Buffer (2X), 15 mL |
| Concentration | 2X |
| Volumes | 4 ml (D5020-9), 15 ml (D5021-9) |
| For use with | EZ DNA Methylation-Direct Kit |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Sample digestion for direct bisulfite conversion
- Blood, tissue, and cell sample preparation
- EZ DNA Methylation-Direct kit replenishment
