Product Description
Desulphonation is the step that turns bisulfite-treated DNA into a usable template, and having enough buffer on the shelf keeps that step from becoming the limiting factor. This replacement buffer is intended for the EZ DNA Methylation-Lightning kits and supports cleanup of converted DNA after the conversion reaction.
This listing is for catalog number D5031-5: L-Desulphonation Buffer, 40 mL. After conversion, DNA is bound to the column and the buffer is applied to remove sulfonate groups while the DNA stays bound, followed by washes and elution. It matches the component supplied in the Lightning kits and is offered in 10 ml, 40 ml, and 80 ml volumes for different kit sizes and throughput levels. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- On-column desulphonation – Performs desulphonation while converted DNA is bound.
- Kit-matched component – Same buffer used in EZ DNA Methylation-Lightning kits.
- Three volumes – Available in 10 ml, 40 ml, and 80 ml sizes.
- Throughput flexibility – Larger volumes support high-throughput formats.
Specifications
| Catalog Number | D5031-5 |
| Brand | Zymo Research |
| Description | L-Desulphonation Buffer, 40 mL |
| Volumes | 10 ml (D5030-5), 40 ml (D5031-5), 80 ml (D5046-5) |
| Function | In-column desulphonation of bisulfite-converted DNA |
| For use with | EZ DNA Methylation-Lightning kits |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Bisulfite DNA cleanup
- EZ DNA Methylation-Lightning kit replenishment
- High-throughput bisulfite workflows
