Product Description
High sample counts no longer have to mean long bisulfite workflows. This 96-well kit brings the speed of the Lightning chemistry to plate format, combining denaturation and conversion into one reaction so that large methylation studies can be processed quickly and consistently with high yields of converted DNA.
This listing is for catalog number D5032: EZ-96 DNA Methylation-Lightning Kit, 2 x 96 Reactions. The ready-to-use Lightning Conversion Reagent is added directly to DNA, and the combined denaturation and conversion step runs without separate preparation. Desulphonation and cleanup are carried out on a 96-well spin plate. The standard kit and the deep-well kit (D5033) each supply 2 x 96 reactions, and the deep-well version allows elution in as little as 15 µl. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Ready-to-use reagent – Lightning Conversion Reagent requires no preparation.
- Combined chemistry – Denaturation and bisulfite conversion occur in a single step.
- 96-well spin plate – Desulphonation and cleanup are performed in plate format.
- Low elution volume – The deep-well kit allows elution in as little as 15 µl.
- NGS-ready output – High-yield converted DNA suits PCR, arrays, and sequencing.
Specifications
| Catalog Number | D5032 |
| Brand | Zymo Research |
| Description | EZ-96 DNA Methylation-Lightning Kit, 2 x 96 Reactions |
| Format | 96-well spin plate; standard (D5032) or deep-well (D5033) |
| Reactions | 2 x 96 |
| Elution volume | As low as 15 µl (deep-well kit) |
| Conversion reagent | Ready-to-use Lightning Conversion Reagent |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- High-throughput methylation profiling
- Methylation arrays
- Bisulfite NGS libraries
- Cohort-scale methylation studies
