Product Description
Antibodies against 5-methylcytosine recognize their target most readily in single-stranded DNA, which makes thorough denaturation an essential first step. This replacement buffer prepares fragmented DNA for immunoprecipitation in the Methylated-DNA IP Kit, so additional samples can be processed without opening a new kit or changing your protocol.
This listing is for catalog number D5101-4-1: DNA Denaturing Buffer, 1 mL. Combine the buffer with fragmented DNA as described in the kit protocol before adding the anti-5-methylcytosine antibody. It is the same component supplied in the Methylated-DNA IP Kit (D5101) and comes as a 1 ml volume. Store between 0 °C and room temperature, and follow the protocol for heating and handling steps. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Denaturation support – Prepares DNA for methylated DNA immunoprecipitation.
- Kit-matched component – Identical to the buffer in the Methylated-DNA IP Kit.
- 1 ml volume – Supplied as a standalone 1 ml tube.
- Simple storage – Store from 0 °C to room temperature.
Specifications
| Catalog Number | D5101-4-1 |
| Brand | Zymo Research |
| Description | DNA Denaturing Buffer, 1 mL |
| Volume | 1 ml |
| Storage | 0 °C to room temperature |
| For use with | Methylated-DNA IP Kit (D5101) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- MeDIP sample preparation
- Methylated DNA enrichment workflows
- Methylated-DNA IP Kit replenishment
