Product Description
Chromatin immunoprecipitation yields tiny amounts of DNA mixed with detergents, antibodies, and enzymes, and losing any of it hurts downstream sensitivity. This spin-column kit recovers clean, concentrated DNA from virtually any stage of a ChIP protocol, giving qPCR and sequencing assays a high-quality template with minimal sample loss.
This listing is for catalog number D5205: ChIP DNA Clean & Concentrator, 50 Preps. A specially formulated ChIP DNA Binding Buffer drives DNA onto the column even when detergents, antibodies, and proteinases are present. Load samples after reverse cross-linking, proteinase K or RNase A treatment, mechanical or nuclease shearing, or elution from chromatin-antibody-bead complexes, then wash and elute in a small volume. Each kit contains 50 preps. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Rapid cleanup – Purifies and concentrates ChIP DNA quickly in a spin-column format.
- Specialized binding buffer – Promotes DNA binding despite detergents, antibodies, and proteinases.
- Any ChIP step – Accepts reverse cross-linked, enzyme-treated, sheared, or bead-eluted samples.
- Concentrated recovery – Elutes DNA in a small volume for sensitive downstream assays.
- 50 preps – Supplied in a 50-prep kit.
Specifications
| Catalog Number | D5205 |
| Brand | Zymo Research |
| Description | ChIP DNA Clean & Concentrator, 50 Preps |
| Preps | 50 |
| Format | Spin column |
| Compatible samples | Reverse cross-linked, proteinase K or RNase A treated, sheared, and bead-eluted ChIP DNA |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- ChIP-qPCR
- ChIP-seq library input
- Sheared chromatin DNA cleanup
- Cross-link reversal cleanup
