Product Description
Mapping where transcription factors and regulatory proteins sit on the genome requires capturing those interactions exactly as they occur in the cell. This chromatin immunoprecipitation kit offers a streamlined procedure for studying cross-linked protein-DNA complexes and identifying binding sites for transcription factors, co-factors, and other DNA regulatory proteins.
This listing is for catalog number D5210: Zymo-Spin ChIP Kit, 25 Preps. Cells are cross-linked to fix proteins on their DNA targets, nuclei are prepared, and chromatin is sheared before immunoprecipitation with a user-supplied ChIP-grade antibody and Protein A beads. Sequential wash buffers remove nonspecific material, then the chromatin is eluted, reverse cross-linked, and purified on spin columns. The kit is offered in 10- and 25-prep sizes. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Native interactions – Captures protein-DNA binding fixed in its natural state.
- Complete buffer set – Includes buffers for nuclei prep, shearing, dilution, washing, and elution.
- Antibody flexible – Works with your own ChIP-grade antibody.
- Spin column purification – Recovers ChIP DNA in a column format.
- Two sizes – Available as 10 or 25 preps.
Specifications
| Catalog Number | D5210 |
| Brand | Zymo Research |
| Description | Zymo-Spin ChIP Kit, 25 Preps |
| Preps | 10 (D5209), 25 (D5210) |
| Antibody | User-supplied ChIP-grade antibody |
| Capture | Protein A beads |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
| Note | Product contains multiple components packaged separately |
Available Sizes & Components
Applications
- ChIP-qPCR
- ChIP-seq
- Transcription factor binding site mapping
- Histone modification analysis
- Gene regulation studies
