Product Description
Uniform DNA attachment to the plate is what makes ELISA wells comparable from one sample to the next. This coating buffer is the replacement component for the Quest 5-hmC DNA ELISA Kit, used to bind sample and control DNA to wells before detection of DNA hydroxymethylation.
This listing is for catalog number D5425-1-30: Coating Buffer, 30 mL. Prepare DNA in the buffer and apply it to the plate as described in the Quest 5-hmC DNA ELISA protocol, then continue with the antibody and development steps. The buffer is identical to the kit component and is available in 15 ml and 30 ml sizes for single or larger assay runs. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Plate coating – Binds sample and control DNA to ELISA wells.
- Kit-matched component – Same buffer supplied in the Quest 5-hmC DNA ELISA Kit.
- Two volumes – Available in 15 ml and 30 ml sizes.
- Sold separately – Restock without purchasing a full kit.
Specifications
| Catalog Number | D5425-1-30 |
| Brand | Zymo Research |
| Description | Coating Buffer, 30 mL |
| Volumes | 15 ml (D5425-1-15), 30 ml (D5425-1-30) |
| For use with | Quest 5-hmC DNA ELISA Kit |
| Shipping Conditions | Ships on blue ice |
| Storage | 4°C |
| Note | Product contains multiple components shipped separately. Blue ice items and dry ice items must have separate ice boxes |
Available Sizes & Components
Applications
- 5-hmC ELISA plate coating
- DNA hydroxymethylation quantification
- Quest 5-hmC ELISA Kit replenishment
