Product Description
Fungal community profiling gets much easier when ITS library prep takes minutes instead of hours. This Zymo Research kit targets the ITS region with a streamlined, automation-friendly workflow and unique dual indexing, cutting hands-on time to about 30 minutes while improving barcode resolution in multiplexed runs.
This listing is for catalog number D6425-PS1: Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 1. Specially designed primers amplify the ITS target and attach barcodes in a single qPCR/PCR reaction. Libraries are pooled by equal volume and cleaned once as a pool, avoiding repeated bead-based purifications, and no TapeStation or gel quantification is needed. Each kit covers 96 preps, with four primer sets available for scaling up to larger fungal and mycobiome studies. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- ITS region targeting – Amplifies the fungal ITS region for mycobiome profiling.
- Unique dual indexing – UDI barcodes improve sample resolution in multiplexed runs.
- Single-PCR workflow – Amplification and barcode addition happen in one qPCR/PCR step.
- One final clean-up – Pool by equal volume and purify the combined library once.
- About 30 minutes hands-on – No separate TapeStation or gel quantification required.
- Automation-friendly – Suited to liquid-handling platforms.
Specifications
| Catalog Number | D6425-PS1 |
| Brand | Zymo Research |
| Description | Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 1 |
| Target region | ITS |
| Indexing | Unique dual indexes (UDI) |
| Preps | 96 preps per kit |
| Primer sets | Primer Sets 1-4 available |
| Hands-on time | About 30 minutes |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Available Sizes & Components
- D6425-PS2 – Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 2
- D6425-PS3 – Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 3
- D6425-PS4 – Quick-ITS Plus NGS Library Prep Kit (UDI) (96 preps) with Primer Set 4
Applications
- Fungal ITS amplicon sequencing
- Mycobiome profiling
- Environmental fungal surveys
- Automated NGS library preparation
