Product Description
Species-level microbiome resolution often requires reading the entire 16S gene. This Zymo Research kit delivers pooled, purified full-length 16S libraries ready for PacBio and Oxford Nanopore sequencing in as little as 30 minutes of hands-on time, without ice buckets or cold blocks. It is built for labs that want long-read taxonomic detail at scale.
This listing is for catalog number D6450-PS2: Quick-16S Full-Length Library Prep Kit with Primer Set 2. Premixed 96-well reagent plates let users simply add DNA and amplify. Four uniquely barcoded plate formats are available, allowing up to 384 samples to be multiplexed. The PCR premix is qPCR compatible, includes enough dead volume for automated liquid handling and uses highly stable reagents so reactions can be set up at room temperature. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Full-length 16S – Produces libraries spanning the whole 16S gene for long-read platforms.
- Premixed 96-well plates – Add DNA and amplify with minimal setup.
- Multiplex up to 384 samples – Four uniquely barcoded formats are available.
- Room-temperature setup – Stable reagents remove the need for ice or cold blocks.
- Automation-ready – Sufficient dead volume supports liquid handlers.
- qPCR compatible – Premix allows amplification to be monitored in real time.
Specifications
| Catalog Number | D6450-PS2 |
| Brand | Zymo Research |
| Description | Quick-16S Full-Length Library Prep Kit with Primer Set 2 |
| Platforms | PacBio, Oxford Nanopore |
| Format | 96-well premixed reagent plates |
| Primer sets | Primer Sets 1-4 available |
| Multiplexing | Up to 384 samples |
| Hands-on time | As little as 30 minutes |
| Setup | Room temperature |
| Shipping Conditions | Ships on dry ice |
| Storage | -20C/4C |
| Note | Product contains multiple components shipped separately. (Blue ice and Dry ice) |
Available Sizes & Components
- D6450-PS1 – Quick-16S Full-Length Library Prep Kit with Primer Set 1
- D6450-PS3 – Quick-16S Full-Length Library Prep Kit with Primer Set 3
- D6450-PS4 – Quick-16S Full-Length Library Prep Kit with Primer Set 4
Applications
- Full-length 16S sequencing on PacBio
- Full-length 16S sequencing on Oxford Nanopore
- Species-level microbiome profiling
- Automated long-read library preparation
