Product Description
Breaking down yeast and fungal cell walls is the first step in spheroplasting, transformation, immunofluorescence and protein purification. This Zymo Research lytic enzyme from Arthrobacter luteus gives researchers a dependable way to digest those walls, packaged with a buffer tuned for maximum activity. It remains a staple reagent in yeast genetics labs.
This listing is for catalog number E1005: Zymolyase, 2000 U. Zymolyase works mainly through beta-1,3 glucanase and beta-1,3-glucan laminaripentao-hydrolase activities, cleaving beta-1,3-glucan linkages and releasing laminaripentaose. It performs best at 30 to 37°C, and activity stops at higher temperatures. The enzyme is lyophilized and offered as 1000 U or 2000 U, plus R-Zymolyase, which adds 0.5 U/µl RNase A on reconstitution. Many yeast genera, including Saccharomyces, Candida and Pichia, are susceptible. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Cell wall digestion – Hydrolyzes beta-1,3-glucan linkages in yeast and fungal walls.
- Optimized buffer – Supplied with a resuspension buffer formulated for maximal activity.
- Defined temperature range – Optimal activity at 30 to 37°C, ceasing at higher temperatures.
- RNase A option – R-Zymolyase provides 0.5 U/µl RNase A once reconstituted.
- Broad susceptibility – Effective on Saccharomyces, Candida, Pichia, Kluyveromyces and many other genera.
Specifications
| Catalog Number | E1005 |
| Brand | Zymo Research |
| Description | Zymolyase, 2000 U |
| Source | Arthrobacter luteus |
| Form | Lyophilized, with storage/resuspension buffer (500 µl) |
| Sizes | 1000 U; 2000 U; R-Zymolyase 1000 U with RNase A |
| Optimal temperature | 30°C - 37°C |
| Main activities | Beta-1,3 glucanase; beta-1,3-glucan laminaripentao-hydrolase |
| R-Zymolyase | Includes 0.5 U/µl RNase A when reconstituted |
| Shipping Conditions | Ships at room temperature |
| Storage | -20°C |
Available Sizes & Components
Applications
- Yeast spheroplast preparation
- Yeast transformation
- Immunofluorescence of yeast cells
- Protein and nucleic acid extraction from yeast
