Product Description
Counter-selection against URA3 is one of the most useful tools in yeast genetics, and solubility problems with powdered 5-FOA should not slow it down. Zymo Research offers 5-FOA both as a ready 100X solution in DMSO and as a synthetic monohydrate powder, each validated for counter-selection activity.
This listing is for catalog number F9003: 100X 5-Fluoroorotic Acid (Liquid), 10 mL. 5-FOA is harmless to yeast until a functional URA3 gene, which encodes orotidine-5'-monophosphate decarboxylase, converts it into toxic 5-fluorouracil. Only Ura- cells survive, enabling plasmid curing, plasmid shuffling, allelic replacement and two-hybrid screens. Choose 1 g or 5 g powder, or a 10 ml bottle of 100 mg/ml liquid concentrate that avoids water-solubility issues. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- URA3 counter-selection – Selects for Ura- yeast cells that lose URA3 function.
- Liquid option – 100X (100 mg/ml) solution in DMSO avoids solubility problems.
- Powder option – Synthetic 5-FOA monohydrate in 1 g and 5 g sizes.
- Activity validated – Both formats tested for counter-selection performance.
- Versatile screening – Supports plasmid shuffling, curing, allelic replacement and two-hybrid assays.
Specifications
| Catalog Number | F9003 |
| Brand | Zymo Research |
| Description | 100X 5-Fluoroorotic Acid (Liquid), 10 mL |
| Formats | Powder (1 g, 5 g); 100X liquid in DMSO (10 ml) |
| Liquid concentration | 100 mg/ml (100X) |
| Mechanism | Converted to 5-fluorouracil in URA3-expressing strains |
| Validation | Tested for counter-selection activity |
| Shipping Conditions | Ships on blue ice |
| Storage | -20°C |
Available Sizes & Components
Applications
- Yeast plasmid shuffling
- Plasmid curing
- Allelic replacement
- Yeast two-hybrid screening
- URA3 counter-selection
