Product Description
Getting more colonies from every transformation starts well before the DNA goes in. This growth medium from Zymo Research is formulated specifically for culturing E. coli that will be made competent, and it typically raises transformation efficiency roughly 5 to 100 fold over conventional SOB, with the exact gain depending on the strain you work with.
This listing is for catalog number M3015-500: ZymoBroth, 500 mL. Grow your starter culture in ZymoBroth, then carry the cells through your competent-cell protocol. It is the same medium used in the Mix & Go! E. coli Transformation Kit, so labs can prepare their own batches of Mix & Go! cells in house. Testing has shown improved efficiency across every strain evaluated, including K12 derivatives such as JM109 and HB101 and B strain derivatives such as BL21. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Higher transformation efficiency – Typically improves efficiency about 5 to 100 fold compared with standard SOB medium.
- Broad strain compatibility – Shown to boost results in all E. coli strains tested, from K12 lines to BL21-type B strains.
- Make your own competent cells – Lets you produce homemade Mix & Go! E. coli cells for routine cloning.
- Purpose-built formulation – Designed specifically for growing cells destined for competent-cell preparation.
- Two pack sizes – Offered in 100 ml and 500 ml bottles to match occasional or high-volume use.
Specifications
| Catalog Number | M3015-500 |
| Brand | Zymo Research |
| Description | ZymoBroth, 500 mL |
| Format | Liquid growth medium |
| Sizes | 100 ml; 500 ml |
| Efficiency gain | Typically 5–100 fold vs. SOB medium (strain dependent) |
| Tested strains | K12 derivatives (e.g., JM109, HB101) and B strain derivatives (e.g., BL21) |
| Companion product | Mix & Go! E. coli Transformation Kit |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Preparation of chemically competent E. coli
- Plasmid cloning and subcloning
- Library construction transformations
- Protein expression strain preparation (BL21)
- Routine bacterial culture for transformation
