Product Description
The MTC Bio OptiWell™ D3096-45S is a 96-well deep well plate with square V-bottom wells and 2.3mL capacity per well — sterile by gamma irradiation, virgin medical-grade polypropylene, black printed alpha-numerics, minimal binding and liquid retention, compatible with all popular automated research platforms and robotic equipment. OptiWell plates come in 24, 48, 96, or 384 format for virtually every research lab, HTS application, and automated/robotic platform. These plates are useful for bacterial cultures, centrifugation (up to 4,000 × g) and general sample storage. Plates are produced and packaged in a Class 100,000 clean room and are ANSI/SLAS 1-2004 compliant. All plates are certified non-pyrogenic and RNase/DNase free. Sterilized by gamma irradiation. Case of 50 plates — 10 bags of 5 plates each.
Square V-Bottom Wells — Maximum Sample Recovery at the Largest 96-Well Deep Well Volume The square V-bottom well geometry of the D3096-45S combines the complete sample recovery of a conical V-bottom with the maximum volume capacity of a square well cross-section — concentrating sample, beads, or pellets to the absolute lowest point of each well for complete recovery by pipette tip or robotic probe, while the square cross-section delivers the largest possible volume within the ANSI/SLAS standard 96-well footprint. At 2.3mL — the largest volume in the LIGHT LABS OptiWell™ lineup — the square V-bottom geometry is especially valuable for applications requiring complete recovery from large-volume reactions, bead-based workflows with substantial buffer volumes, and any sterile workflow where maximum well volume combined with complete sample recovery are both required simultaneously.
2.3mL Per Well — The Largest Volume in the 96-Well Deep Well Format The 2.3mL per-well capacity of the D3096-45S is the largest volume in the 96-well deep well format available in the LIGHT LABS OptiWell™ lineup — 0.1mL larger than the 2.2mL U-bottom formats (D3096-41S and D3096-43S) and providing the absolute maximum working volume for sterile bacterial culture, protein expression, bead-based extraction, and large-volume sterile compound preparation workflows. For labs whose protocols are specifically optimized at 2.3mL scale, or whose overnight bacterial culture volumes require slightly more medium than the 2.2mL format provides, the D3096-45S is the precisely correct sterile format.
D3096-45S vs. D3096-41S and D3096-43S — Square V-Bottom vs. Square U-Bottom at ~2.2mL Sterile:
| D3096-45S | D3096-41S | D3096-43S | |
|---|---|---|---|
| Well Bottom | V-bottom | U-bottom Standard | U-bottom H-style |
| Volume | 2.3mL | 2.2mL | 2.2mL |
| Best For | Max recovery + max volume in sterile format | Sterile general automation | KingFisher™ magnetic bead, sterile |
| Sterile | Yes | Yes | Yes |
| Case Qty | 50 | 50 | 50 |
The D3096-45S square V-bottom format is the correct choice when both maximum sample recovery and maximum well volume are simultaneously required in a sterile format — a combination not available in the U-bottom formats.
Sterile by Gamma Irradiation — Validated for Sterile Workflows Sterile. Sterilized by gamma irradiation. The D3096-45S is sterilized by gamma irradiation — providing validated, reliable sterility without heat damage or residual chemical concerns. The "S" suffix designates the sterile format. Gamma irradiation sterilization qualifies the D3096-45S for sterile bacterial culture, protein expression, sterile compound preparation, and any sterile 96-well deep well application requiring square V-bottom geometry at maximum 2.3mL capacity.
D3096-45S in the Full OptiWell™ 96-Well Deep Well Family:
| Cat. # | Wells | Bottom | Volume | Sterile | Case Qty |
|---|---|---|---|---|---|
| D3096-09 | 96 Square | V-bottom | 0.5mL | No | 100 |
| D3096-09S | 96 Square | V-bottom | 0.5mL | Yes | 100 |
| D3096-19 | 96 Square | V-bottom | 1.0mL | No | 100 |
| D3096-19S | 96 Square | V-bottom | 1.0mL | Yes | 100 |
| D3096-41 | 96 Square | U-bottom Standard | 2.2mL | No | 50 |
| D3096-41S | 96 Square | U-bottom Standard | 2.2mL | Yes | 50 |
| D3096-43 | 96 Square | U-bottom H-style | 2.2mL | No | 50 |
| D3096-43S | 96 Square | U-bottom H-style | 2.2mL | Yes | 50 |
| D3096-45S | 96 Square | V-bottom | 2.3mL | Yes | 50 |
Automated Platform Compatibility — ANSI/SLAS 1-2004 Standard For automated research platforms and robotic equipment. The D3096-45S conforms to ANSI/SLAS 1-2004 standard 96-well microplate footprint dimensions — ensuring compatibility with all major automated liquid handling platforms including Hamilton, Tecan, Beckman Coulter, PerkinElmer, Agilent, and other standard HTS robotic systems.
4,000 × g Centrifugation Rating Centrifugation up to 4,000 × g — covering all standard plate centrifugation protocols for sterile bacterial cell pelleting, bead collection, and sample concentration at 2.3mL scale.
Virgin Medical-Grade Polypropylene — Minimal Binding and Liquid Retention Minimal binding / liquid retention. Virgin, medical-grade polypropylene. Certified RNase/DNase free and non-pyrogenic.
Black Printed Alpha-Numerics — Instant Well Identification Black printed alpha-numerics. Molded black alpha-numeric well identification (A through H rows, 1 through 12 columns).
Class 100,000 Cleanroom Production — Certified Purity Plates are produced and packaged in a Class 100,000 clean room. Certified non-pyrogenic and RNase/DNase free.
Compatible Replacement — Cross-Reference Guide
The MTC Bio OptiWell™ D3096-45S is a comparable alternative to sterile 96-well square V-bottom large-volume deep well plates from several major manufacturers:
- Thermo Fisher AB-0564 — Thermo Scientific 96-well square V-bottom deep well plate, 2.2mL, sterile
- Greiner Bio-One 780270 — 96-well deep well plate, square V-bottom, 2.2mL, sterile
- Eppendorf® 0030501344 — Eppendorf 96-well deepwell plate, square V-bottom, 2.2mL, sterile
Competitor catalog numbers are referenced for cross-referencing purposes only. Volume specifications may vary. Compatibility should be verified for your specific application and protocol.
Key Features
- MTC Bio OptiWell™ — Catalog # D3096-45S
- 96-well deep well plate — square V-bottom wells
- 2.3mL per-well capacity — largest volume in the LIGHT LABS OptiWell™ 96-well lineup
- Square V-bottom — maximum sample recovery at maximum volume in sterile format
- Sterile — gamma irradiation
- Virgin medical-grade polypropylene — minimal binding and liquid retention
- Black printed alpha-numerics — instant well identification
- ANSI/SLAS 1-2004 compliant — compatible with all standard automated liquid handling platforms
- 4,000 × g centrifugation rating
- Compatible with silicone sealing mats and sealing film
- No lid, no skirt
- Class 100,000 cleanroom production
- Certified RNase-free and DNase-free
- Certified non-pyrogenic
- Sigma-Aldrich catalog # MTCD309645S
- Case of 50 plates — 10 bags of 5 plates
Supported Applications Sterile bacterial liquid culture at maximum 96-well deep well volume, sterile protein expression in E. coli at 2.3mL scale, sterile large-volume bead-based assay preparation requiring V-bottom complete recovery, sterile compound preparation and dilution at 2.3mL scale, sterile automated liquid handling at maximum deep well volume, centrifugation workflows up to 4,000 × g, and any 96-well application requiring sterile square V-bottom deep well format with 2.3mL maximum per-well capacity and full automated platform compatibility.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Catalog # | D3096-45S |
| Sigma-Aldrich Catalog # | MTCD309645S |
| Manufacturer | MTC Bio |
| Product Line | OptiWell™ |
| Well Format | 96-well |
| Well Shape | Square |
| Well Bottom | V-bottom (conical) |
| Well Volume / Capacity | 2.3mL per well |
| Material | Virgin medical-grade polypropylene |
| Color | Clear |
| Alpha-Numerics | Yes — black printed |
| Lid | No |
| Skirt | No |
| Centrifugation Rating | 4,000 × g |
| Binding | Minimal — low binding and liquid retention |
| Sealing Compatibility | Silicone sealing mats and sealing film |
| Sterility | Sterile — gamma irradiation |
| Footprint | ANSI/SLAS 1-2004 compliant |
| Production Environment | Class 100,000 cleanroom |
| RNase-Free | Yes — certified |
| DNase-Free | Yes — certified |
| Non-Pyrogenic | Yes — certified |
| Case Quantity | 50 plates (10 bags × 5 plates) |