MTC Bio T9009 FACS Cell Strainer Cap, 35µm Nylon Mesh, Green, Sterile — 500/Pack (20 Bags of 25)

Product Description

The MTC Bio T9009 is a 35µm FACS cell strainer cap for use with 5mL 12×75mm FlowTubes™ and standard FACS tubes — ideal for detaching, isolating and straining cells from clusters and tissues. Their built-in compatibility with 12×75mm culture tubes and FACS tubes makes them especially useful for pre-sorting and preparing samples for Fluorescence-activated Cell Sorting (FACS). Strainer cap contains 35µm nylon mesh screen. Assists in cell sorting/straining applications. For use with FlowTubes 12x75mm culture tubes. Supplied in pack of 500 (20 bags of 25 caps). LDPE dual-position cap. Green color coded by pore size. E-Beam sterilized to SAL 10&sup6;. Sterile pouches of 25 strainer caps. Also available as T9009-35. 

T9009 = T9009-35 — Same Product, Two Catalog Numbers The MTC Bio T9009 and T9009-35 are the same product — the 35µm green FACS cell strainer cap. Fisher Scientific, Sigma-Aldrich, and other major distributors list this item under both catalog numbers. Labs sourcing this cap under either T9009 or T9009-35 will receive identical product. LIGHT LABS stocks this item as the T9009-35 — both catalog numbers are fully searchable and will find this listing.

35µm Mesh — The Standard Pore Size for Single-Cell Suspension Preparation Typically a 35µm porous mesh is used to obtain a single-cell suspension and to prevent clogging in FACS instrumentation. The 35µm pore size is the most widely used strainer mesh size for flow cytometry sample preparation — large enough to allow single cells to pass freely while retaining tissue aggregates, clumps, and debris that would clog the instrument sample injection needle. For most flow cytometry workflows involving dissociated tissue, single-cell suspensions, or any heterogeneous cell sample requiring pre-sorting filtration, the T9009 35µm is the standard first choice.

Dual-Position LDPE Cap — Culture and Close in the Same Cap LDPE Bio Strainer caps are ideal for detaching, isolating and straining cells from clusters and tissues. Their built-in compatibility with 12×75mm culture tubes and FACS tubes makes them especially useful for pre-sorting and preparing samples for Fluorescence-activated Cell Sorting (FACS). The dual-position design allows the strainer cap to function in both a vented culture position — allowing gas exchange during incubation — and a fully closed position for sample containment during transport and centrifugation. This eliminates the need to switch caps between workflow steps.

Compatible with BD FACS Instruments and All Standard 12×75mm Tubes Compatible with FACS (BD) instruments. Clear polystyrene, 5mL capacity. Available with 35µm strainer cap, standard cap, or no cap. The T9009 fits securely on all standard 5mL 12×75mm round-bottom polystyrene FACS tubes — including MTC Bio FlowTubes™, Falcon 352058, and equivalent formats from all major manufacturers, and is specifically designed for compatibility with BD FACSCanto, FACSAria, FACSVerse, and other BD Biosciences FACS instruments.

Green Color Coding — Instant 35µm Pore Size Identification Color coded by pore size. Green is the designated color for the 35µm pore size across the MTC Bio T9009 strainer cap family — allowing instant visual identification of the correct strainer mesh size in busy flow cytometry lab environments where multiple pore sizes may be in simultaneous use. 

E-Beam Sterilized to SAL 10&sup6; — Validated Sterility Each cap is E-Beam sterilized (SAL 10&sup6;) and comes in pouches of 25. Pack Size: 500 caps total, supplied in 20 bags of 25 caps each. E-beam sterilization provides validated, reliable sterility without heat damage or residual chemical concerns — qualifying the T9009 for the most sensitive cell sorting and sterile FACS applications.

Complete MTC Bio T9009 Cell Strainer Cap Family — All Pore Sizes:

Compatible Replacement — Cross-Reference Guide

The MTC Bio T9009 is a direct compatible replacement for 35µm FACS cell strainer caps from several major manufacturers:

• BD Biosciences 352235 — BD Falcon 5mL tube with cell strainer cap, 35µm
• pluriSelect 43-10035-40 — 35µm cell strainer cap for 5mL FACS tubes

Competitor brand names and catalog numbers are referenced for cross-referencing purposes only. Compatibility should be verified for your specific tubes and protocol.

Key Features

• MTC Bio T9009 / T9009-35 FACS cell strainer cap — same product, two catalog numbers
• 35µm nylon mesh — standard pore size for single-cell suspension and FACS pre-sorting
• Green color coded — instant 35µm pore size identification
• Dual-position LDPE cap — vented culture position and closed position
• Compatible with BD FACS instruments — FACSCanto, FACSAria, FACSVerse, and others
• Fits all standard 5mL 12×75mm FACS tubes and culture tubes
• Compatible with MTC Bio FlowTubes™ and Falcon 352058 equivalent formats
• E-Beam sterilized to SAL 10&sup6; — validated sterility
• Sterile pouches of 25 caps — convenient session-sized access
• 500 caps per pack — 20 bags of 25 caps
• Fisher Scientific catalog # NC1827548

Supported Applications Flow cytometry sample preparation, FACS single-cell suspension preparation, cell straining and debris removal before flow cytometer loading, dissociated tissue single-cell suspension filtration, bone marrow and spleen single-cell preparation, tumor dissociation filtration, peripheral blood mononuclear cell preparation, high-throughput FACS sorting sample pre-filtration, and any flow cytometry or cell sorting application requiring 35µm filtration of a cell suspension before instrument loading.

Technical Specifications