Product Description
The final step of a His-tag purification determines how much protein you actually recover. This Zymo Research elution buffer is the release reagent from the His-Spin Protein Miniprep, sold separately so you can keep purifying histidine-tagged proteins on schedule without reordering the full kit or waiting on a backorder.
This listing is for catalog number P2003-5: His-Elution Buffer, 25 mL. After binding and washing, the elution buffer frees the His-tagged target from the nickel affinity gel so it can be collected in a small volume ready for downstream analysis. It is intended for use with His-Affinity Gel, His-Binding Buffer and His-Wash Buffer in the kit's spin-column protocol. Supplied in a 25 ml bottle. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Kit component – The elution buffer from the His-Spin Protein Miniprep (P2001).
- Releases tagged protein – Frees His-tagged targets from nickel affinity gel.
- Matched system – Works with His-Affinity Gel and companion buffers.
- Simple restocking – Replace elution buffer independently of the kit.
- 25 ml bottle – Convenient quantity for multiple preps.
Specifications
| Catalog Number | P2003-5 |
| Brand | Zymo Research |
| Description | His-Elution Buffer, 25 mL |
| Volume | 25 ml |
| Compatible kit | His-Spin Protein Miniprep (P2001) |
| Shipping Conditions | Ships at room temperature |
| Storage | 4°C |
Applications
- His-tagged protein elution
- His-Spin Protein Miniprep refills
- Nickel affinity purification
- Recombinant protein workflows
