Product Description
Humic acids, tannins and tough microbial cell walls can derail RNA work from environmental and stool samples. This Zymo Research microprep kit combines bead-beating lysis with dedicated inhibitor removal, delivering total RNA, small RNAs included, from soil, sludge, sediment and feces in a form that performs reliably in RT-PCR.
This listing is for catalog number R2040: ZR Soil/Fecal RNA MicroPrep, 50 Preps. Samples go into a ZR BashingBead Lysis Tube with S/F RNA Lysis Buffer, where bead beating breaks open bacteria, fungi, protists and algae. Zymo-Spin columns then filter the lysate, remove genomic DNA and capture RNA. A Zymo-Spin III-HRC Spin Filter clears PCR inhibitors, and the RNA is concentrated on a Zymo-Spin IC Column for elution in as little as 6 µl. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Mechanical lysis – bead beating in ZR BashingBead Lysis Tubes opens tough-to-lyse microbes.
- Inhibitor removal – the Zymo-Spin III-HRC Spin Filter removes humic acids, polyphenols and tannins.
- Genomic DNA removal – column-based filtration clears DNA from the lysate before RNA binding.
- Small RNA recovery – isolates total RNA including small RNAs longer than 17 nt.
- Low elution volume – the Zymo-Spin IC Column concentrates RNA into 6 µl or more.
Specifications
| Catalog Number | R2040 |
| Brand | Zymo Research |
| Description | ZR Soil/Fecal RNA MicroPrep, 50 Preps |
| Compatible samples | Soil, sludge, sediment and fecal samples |
| RNA recovered | Total RNA including small RNAs (>17 nt) |
| Lysis method | Bead beating in ZR BashingBead Lysis Tubes with S/F RNA Lysis Buffer |
| Inhibitor removal | Zymo-Spin III-HRC Spin Filter (humic acids, polyphenols, tannins) |
| Minimum elution volume | 6 µl |
| Kit size | 50 preps |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- Soil and sediment metatranscriptomics
- Gut microbiome RNA studies
- Wastewater and sludge microbial analysis
- RT-qPCR detection of microbial targets
