Product Description
Map RNA cytosine methylation without precipitation headaches. This Zymo Research kit performs fast, reliable bisulfite conversion of RNA, turning unmethylated cytosines into uracil while protecting templates from degradation and loss, for accurate methylation analysis. It brings bisulfite-based methylation analysis, a staple of DNA epigenetics, to RNA studies with a protocol that is easy to adopt.
This listing is for catalog number R5001: EZ RNA Methylation Kit, 50 Preps. Denaturation and bisulfite conversion are combined into one step using a ready-to-use RNA Conversion Reagent, so no buffer preparation is needed. Desulphonation then takes place on the column, removing messy precipitation steps and improving consistency. Recovered RNA is suitable for RT-PCR, sequencing and NGS library preparation. Kits are available in 50- and 200-prep sizes. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Streamlined conversion – RNA denaturation and bisulfite conversion happen in a single step.
- Ready-to-use reagent – no buffer preparation before starting.
- In-column desulphonation – eliminates precipitation for more consistent results.
- Template protection – designed to minimize RNA degradation and loss.
- Complete conversion – thorough cytosine conversion supports accurate methylation calls.
Specifications
| Catalog Number | R5001 |
| Brand | Zymo Research |
| Description | EZ RNA Methylation Kit, 50 Preps |
| Method | RNA bisulfite conversion |
| Desulphonation | In-column |
| Downstream | RT-PCR, sequencing, library preparation, NGS |
| Kit sizes | 50 or 200 preps |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- RNA m5C methylation analysis
- Bisulfite RNA sequencing
- Epitranscriptomics research
- RT-PCR of converted RNA
