Product Description
Turn the E. coli strains already in your freezer into highly competent cells, with no heat shock required. This Zymo Research system makes transformation as simple as mixing DNA with cells and spreading onto a plate, with efficiencies typically reaching 10^8 to 10^9 transformants per µg of plasmid.
This listing is for catalog number T3002: Mix & Go! E.coli Transformation Buffer Set, up to 60 mL. Grow your chosen strain, wash, and resuspend the cells in the supplied buffers; they are then ready to transform. The Transformation Kit includes all buffers plus ZymoBroth medium to make 20 ml of competent cells. The Buffer Set provides buffers for 60 ml of competent cells, with growth medium supplied by the user. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- No heat shock – transform by simply mixing DNA with cells and plating.
- High efficiency – typically 10^8 to 10^9 transformants per µg plasmid DNA with most strains.
- Use your own strains – converts lab E. coli strains into competent cells.
- Simple prep – grow, wash and resuspend in the provided buffers.
- Two formats – kit with ZymoBroth for 20 ml, or buffer set for 60 ml of cells.
Specifications
| Catalog Number | T3002 |
| Brand | Zymo Research |
| Description | Mix & Go! E.coli Transformation Buffer Set, up to 60 mL |
| Transformation efficiency | Typically 10^8-10^9 transformants/µg plasmid DNA |
| Heat shock | Not required |
| Transformation Kit yield | Up to 20 ml competent cells (buffers and ZymoBroth included) |
| Buffer Set yield | Up to 60 ml competent cells (medium supplied by user) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Plasmid transformation
- Cloning and subcloning
- Making competent cells from custom strains
- Routine plasmid propagation
