Zymo Research ZR Plasmid Miniprep™-Classic D4054 | 800 Preps with Zymo-Spin™ IIN Columns

The Zymo Research ZR Plasmid Miniprep™-Classic D4054 is the 800-preparation large-volume plasmid DNA purification kit — the highest-throughput format in the ZR Plasmid Miniprep™-Classic family, designed for core facilities, high-throughput cloning labs, and any environment where plasmid miniprep kits are consumed in large quantities. Designed for efficient isolation of plasmid DNA from E. coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, and reliable. Color-coded buffers (Red, Green, Yellow) for easy visualization of complete cell lysis and neutralization. Unique Zymo-Spin™ IIN column design with zero buffer retention and low-volume 30µL elution. Transfection-grade plasmid DNA with Endo-Wash Buffer included. ~15 minutes per preparation. Room temperature storage. Catalog # D4054. Smaller kit sizes are available as D4015 (100 preps) and D4016 (400 preps).

800 Preps — Maximum Throughput for High-Volume Plasmid Work The D4054 800-prep format is the definitive choice for labs and core facilities that consume plasmid miniprep kits at high volume — eliminating the frequent kit reordering and replenishment cycles required when using smaller kit sizes. For core facilities processing hundreds of constructs per week, high-throughput cloning labs running parallel screening workflows, and any environment where plasmid miniprep kits are an essential daily consumable, the D4054 provides the maximum supply efficiency available in the ZR Plasmid Miniprep™-Classic line. At 800 preparations, the D4054 represents the lowest per-prep reagent cost in the family — making it the most economical choice for established, high-volume plasmid isolation workflows.

D4054 vs. D4016 vs. D4015 — Choosing the Right Kit Size:

Color-Coded Buffers — Visual Confirmation of Complete Lysis and Neutralization Innovative colored buffers for rapid identification of complete bacterial cell lysis and neutralization steps. The three-buffer color-coded system of the ZR Plasmid Miniprep™-Classic remains fully intact in the D4054 format — with the same Red (P1), Green (P2), and Yellow (P3) visual endpoints that confirm complete lysis and neutralization at every prep across the full 800-preparation kit. For core facilities and high-throughput labs where multiple operators run preps simultaneously across the day, the color-coded buffer system provides a universal visual quality control that requires no interpretation — complete lysis turns the mixture green when P2 is added, and complete neutralization returns it to yellow when P3 is added.

Zymo-Spin™ IIN Columns — Zero Buffer Retention, 30µL Elution Unique column design: zero buffer retention and low volume (30µl) elution. The zero buffer retention column design and 30µL minimum elution volume are preserved across all kit sizes — ensuring that the highly concentrated, low-volume plasmid DNA eluate of the D4015 100-prep kit is identical in quality and concentration to the eluate from the D4054 800-prep kit. For high-throughput labs where transfection, electroporation, or sequencing reactions are performed at scale, the consistent 30µL concentrated eluate volume across all 800 preparations is essential for maintaining workflow consistency.

Transfection-Grade Plasmid DNA — Endo-Wash Included at Scale Plasmid DNA purified using the ZR Plasmid Miniprep-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc. The Endo-Wash Buffer step is included across the full 800 preparations — ensuring that transfection-grade, low-endotoxin plasmid DNA is produced at every prep throughout the life of the kit. For core facilities running mammalian cell transfections at high throughput, the consistent endotoxin removal performance of the D4054 across 800 preps is a critical quality assurance requirement.

~15 Minutes Per Preparation — Maintained at Scale 15 min prep. The streamlined color-coded protocol completes in approximately 15 minutes per prep — maintained at the same speed in the D4054 800-prep format as in the smaller kit sizes. For core facilities and high-throughput labs processing many preps per day, the consistent 15-minute prep time allows accurate workflow scheduling and capacity planning across the full kit lifetime.

Room Temperature Storage — Complete Kit at Bench Temperature All components including P1 Buffer (Red), P2 Buffer (Green), Endo-Wash Buffer, Plasmid Wash Buffer, DNA Elution Buffer, Zymo-Spin™ IIN Columns, and Collection Tubes are stored at room temperature. P3 Buffer (Yellow) is stored at 4°C after opening. No freezer storage of kit components required — simplifying large-format kit storage in space-limited core facilities and laboratories.

Complete Kit Contents — D4054 (800 Preps):

Compatible Replacement — Cross-Reference Guide

The Zymo Research ZR Plasmid Miniprep™-Classic D4054 is a comparable alternative to large-format plasmid miniprep kits from several major manufacturers:

• Qiagen QIAprep Spin Miniprep Kit (27106) — 250-prep plasmid miniprep kit, alkaline lysis
• Thermo Fisher GeneJET Plasmid Miniprep Kit (K0503) — 250-prep plasmid miniprep kit
• Omega Bio-Tek E.Z.N.A. Plasmid Mini Kit (D6943) — 200-prep plasmid miniprep kit
• Promega PureYield Plasmid Miniprep System (A1224) — high-prep count plasmid miniprep

These products may differ in preparation count, protocol, yield, and performance characteristics. The D4054 provides 800 preparations — the highest throughput format available in the ZR Plasmid Miniprep-Classic line.

Key Features

• Zymo Research ZR Plasmid Miniprep™-Classic — Catalog # D4054
• 800 preparations with Zymo-Spin™ IIN Columns — largest kit size in the family
• Traditional alkaline lysis (P1/P2/P3) — familiar, reliable, validated chemistry
• Color-coded buffers — Red (P1), Green (P2), Yellow (P3) — visual confirmation of lysis and neutralization
• Zero buffer retention Zymo-Spin™ IIN columns — no dry spin required
• 30µL minimum elution volume — highly concentrated plasmid DNA
• 900µL sample capacity per column
• ~15-minute preparation time
• Endo-Wash Buffer included — transfection-grade plasmid DNA
• Endotoxin removal included across all 800 preparations
• Room temperature storage (P3 Buffer at 4°C after opening)
• 1-year kit component integrity guarantee
• Lot-to-lot tested for performance and reliability
• Lowest per-prep reagent cost in the ZR Plasmid Miniprep™-Classic family
• Smaller kit sizes: D4015 (100 preps), D4016 (400 preps)

Supported Applications High-throughput plasmid DNA isolation from E. coli for restriction endonuclease digestion and mapping, Sanger sequencing, DNA ligation and cloning, PCR using plasmid template, bacterial transformation, mammalian cell transfection, electroporation, in vitro transcription, CRISPR vector preparation, colony screening at scale, core facility plasmid isolation services, and any high-volume molecular cloning workflow requiring consistent, transfection-grade plasmid DNA from E. coli in 15 minutes.

Technical Specifications