Product Description
LIGHT LABS is proud to offer the Zymo Research ZymoScript™ One-Step RT-qPCR Kit (Cat # R3014) — an optimized, all-in-one one-step RT-qPCR kit that combines fast, efficient cDNA synthesis with robust qPCR amplification in a single tube for the simplest, fastest path from RNA template to quantitative result. The ZymoScript™ One-Step RT-qPCR Kit contains all necessary components for complete reverse transcription and specific qPCR amplification in as little as 1.5 hours — eliminating the tube transfers, additional reagent additions, and extra incubation steps of two-step RT-qPCR workflows.
A key differentiator of the ZymoScript™ One-Step RT-qPCR Kit is its dual chemistry compatibility — the kit is optimized for use with both SYBR Green intercalating dye and TaqMan probe-based detection, providing ultimate flexibility for labs running multiple assay chemistries on the same instrument or transitioning between detection methods. The included Fluor Dye solution is added directly to the reaction for SYBR Green-based detection, while TaqMan probe-based assays are run without the Fluor Dye for probe-specific signal. This dual compatibility eliminates the need to maintain separate one-step RT-qPCR kits for different assay chemistries.
The unique Reaction Buffer (4X) contains dNTPs, MgCl₂, and proprietary additives that ensure complete cDNA synthesis and specific qPCR amplification in a single streamlined protocol. Temperature-activated enzymes provide a built-in hot start — facilitating melting of secondary structures for efficient reverse transcription while ensuring ultimate specificity through enzyme activation only at higher temperatures. Reactions can be assembled at room temperature without risk of non-specific product formation. A non-interfering blue tracking dye is included in the kit to simplify reaction setup, prevent pipetting errors, and confirm that all wells have been loaded — a practical advantage in high-throughput setups. Note: only gene-specific primers are compatible with this kit (not random hexamers or oligo dT). Genesee Scientific catalog number is 11-735. LIGHT LABS is your trusted source for Zymo Research products.
KEY FEATURES
- One-step RT-qPCR — complete cDNA synthesis and robust qPCR amplification in a single tube in as little as 1.5 hours; no tube transfers between RT and qPCR steps
- Dual chemistry compatibility — compatible with both SYBR Green (using included Fluor Dye) AND TaqMan probe-based assays; one kit for both detection chemistries
- Fast: 1.5 hour complete protocol — from RNA template to quantitative CT result
- Wide RNA input range: 0.1 pg – 5 µg total RNA — sensitive enough for low-abundance targets; accommodates high-input samples
- Temperature-activated hot start enzymes — built-in specificity; room temperature reaction assembly without non-specific product formation
- Non-interfering blue tracking dye — simplifies reaction setup, prevents pipetting errors, confirms well loading in high-throughput applications
- Unique buffer system with temperature-activated enzymes — facilitates melting of RNA secondary structures; ensures complete cDNA synthesis from GC-rich and structured templates
- RT-qPCR product contains "A" overhang — compatible with TA cloning for product verification
- Ideal for pathogen detection, gene expression analysis, and multiplex RT-qPCR assays
- Note: requires gene-specific primers only — not compatible with random hexamers or oligo dT
- 100 reactions per kit
- Storage: -20°C
- Genesee Scientific Catalog No. 11-735
- Made in the USA by Zymo Research
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | R3014 |
| Genesee Scientific Catalog No. | 11-735 |
| Brand | Zymo Research |
| Kit Name | ZymoScript™ One-Step RT-qPCR Kit |
| Number of Reactions | 100 (20 µL reactions) |
| Reaction Volume | 20 µL (recommended) |
| Detection Compatibility | SYBR Green (with included Fluor Dye) AND TaqMan probe-based |
| RNA Input Range | 0.1 pg – 5 µg total RNA |
| Processing Time | ~1.5 hours (complete RT-qPCR) |
| Primer Compatibility | Gene-specific primers only (no random hexamers or oligo dT) |
| Hot Start | Yes — temperature-activated enzymes |
| Reaction Setup Temperature | Room temperature |
| Tracking Dye | Yes — non-interfering blue tracking dye included |
| RT-qPCR Product End | "A" overhang (TA cloning compatible) |
| Equipment Required | Thermal cycler / qPCR instrument |
| Storage Temperature | -20°C |
| Shipping Temperature | Dry ice |
| Shelf Life | 12 months from date of purchase |
| Kit Components | Reaction Buffer 4X (R3014-1-500, 500 µL); Enzyme Mix 20X (R3014-2-100, 100 µL); Fluor Dye 20X (R3014-3-100, 100 µL); MgCl₂ 25 mM (R3014-4-500, 500 µL); Instruction Manual |
SUGGESTED REACTION SETUP (20 µL)
| Component | Volume |
|---|---|
| Reaction Buffer (4X) | 5 µL |
| Enzyme Mix (20X) | 1 µL |
| Fluor Dye (20X) — SYBR Green only | 1 µL |
| Forward Primer (gene-specific) | variable |
| Reverse Primer (gene-specific) | variable |
| RNA Template (0.1 pg – 5 µg) | variable |
| Nuclease-Free Water | to 20 µL |
APPLICATIONS
- Gene expression analysis by one-step RT-qPCR
- Viral RNA detection and quantification (SARS-CoV-2, influenza, HIV, HCV, and other RNA viruses)
- Pathogen detection and infectious disease diagnostics
- mRNA expression profiling from cell culture, tissue, and biological fluid RNA
- Multiplex RT-qPCR assay development
- High-throughput diagnostic RT-qPCR applications
- RT-qPCR from low-abundance RNA targets requiring maximum sensitivity (0.1 pg input)
- Any one-step RT-qPCR application requiring dual SYBR Green/TaqMan compatibility and fast 1.5-hour results
ORDERING INFORMATION — ZYMO RESEARCH ZYMOSCRIPT™ RT-qPCR KITS
| Cat # | Description | Reactions |
|---|---|---|
| R3014 | ZymoScript™ One-Step RT-qPCR Kit | 100 |
| R3012 | ZymoScript™ RT PreMix Kit (2-step RT-qPCR; random hexamer + oligo dT) | 100 |
| R3014-1-500 | Reaction Buffer 4X (individual component) | 500 µL |
| R3014-2-100 | Enzyme Mix 20X (individual component) | 100 µL |
| R3014-3-100 | Fluor Dye 20X — SYBR Green (individual component) | 100 µL |
| R3014-4-500 | MgCl₂ 25 mM (individual component) | 500 µL |
