Product Description
When you need tighter control over the signals your cells receive, this growth factor reduced, phenol red-free matrix offers a cleaner background. Reduced growth factor content and no indicator dye make it well suited to imaging and defined-condition experiments.
This listing is for NEST catalog number 211242: GelNest Basement Membrane Matrix, Growth Factor Reduced, Phenol Red-Free, LDEV-Free, 5 mL, Bottle. GelNest Matrix is prepared from basement membrane components extracted from mouse tumor tissue, rich in laminin, type IV collagen and heparan sulfate proteoglycan along with a range of growth factors. It mimics the physiological basement membrane to provide the adhesion, differentiation and proliferation cues cells need. Every lot is LDEV-free and supplied as 5 mL per bottle; keep it frozen and thaw on ice before use. LIGHT LABS stocks the NEST Scientific line and ships quickly from our Englewood, Colorado team.
Key Features
- Native Basement Membrane Proteins – Laminin, type IV collagen and heparan sulfate proteoglycan support cell adhesion
- LDEV-Free – Tested free of lactate dehydrogenase elevating virus
- Growth Factor Reduced – Lower endogenous signaling for more defined experiments
- Phenol Red-Free – Reduces background in fluorescence and colorimetric assays
- Versatile Gel – Forms a 3D gel for coating or embedding cells
Specifications
| Catalog Number | 211242 |
| Brand | NEST Scientific |
| Description | GelNest Basement Membrane Matrix, Growth Factor Reduced, Phenol Red-Free, LDEV-Free, 5 mL |
| Sold As | Bottle |
| Formulation | Growth factor reduced |
| Phenol Red | No (phenol red-free) |
| Units per Pack | 1 bottle |
| Units per Case | 1 bottle (5 mL) |
| Source | Basement membrane extract from mouse tumor tissue |
| Main Components | Laminin, type IV collagen, heparan sulfate proteoglycan, growth factors |
| Viral Testing | LDEV-free |
| Volume | 5 mL per bottle |
| Storage | Frozen; thaw on ice to avoid premature gelling |
Applications
- Organoid culture under defined conditions
- Fluorescence imaging of 3D cultures
- Stem cell differentiation
- Cell invasion assays
- Hormone-sensitive cell culture
