Product Description
Telling hydroxymethylated DNA apart from methylated or unmodified DNA requires an antibody with real discrimination. This rabbit polyclonal anti-5-hmC antibody separates these cytosine states with little to no cross-reactivity, giving confidence that enrichment and detection signals reflect true 5-hmC. That accuracy matters when mapping this distinct epigenetic mark in research samples.
This listing is for catalog number A4001-200: Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl. Supplied at 1 µg/µl, the antibody has been validated in ELISA and in immunoprecipitation-based enrichment of 5-hmC DNA. It is also suitable for further uses such as immunohistochemical labeling and chromatographic blotting. Use it to quantify global hydroxymethylation, pull down 5-hmC fragments for sequencing or qPCR, or visualize the mark in tissue. Choose 25, 50 or 200 µg. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Strong discrimination – Distinguishes 5-hmC from 5-mC and unmodified cytosine.
- Rabbit polyclonal – Recognizes 5-hydroxymethylcytosine in DNA samples.
- Validated applications – Tested in ELISA and immunoprecipitation enrichment.
- Additional uses – Suitable for immunohistochemistry and chromatographic blotting.
- Convenient sizes – Available in 25, 50 and 200 µg quantities.
Specifications
| Catalog Number | A4001-200 |
| Brand | Zymo Research |
| Description | Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl |
| Host / clonality | Rabbit polyclonal |
| Target | 5-hydroxymethylcytosine |
| Concentration | 1 µg/µl |
| Validated uses | ELISA, immunoprecipitation |
| Pack sizes | 25 µg; 50 µg; 200 µg |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Available Sizes & Components
- A4001-25 – Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl
- A4001-50 – Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl
Applications
- hMeDIP enrichment of 5-hmC DNA
- 5-hmC ELISA quantification
- Immunohistochemical labeling of 5-hmC
- Dot and chromatographic blotting
- Epigenetics research
