Product Description
Telling hydroxymethylated DNA apart from methylated or unmodified DNA requires an antibody with real discrimination. This rabbit polyclonal anti-5-hmC antibody separates these cytosine states with little to no cross-reactivity, giving confidence that enrichment and detection signals reflect true 5-hmC. That accuracy matters when mapping this distinct epigenetic mark in research samples.
This listing is for catalog number A4001-50: Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl. Supplied at 1 µg/µl, the antibody has been validated in ELISA and in immunoprecipitation-based enrichment of 5-hmC DNA. It is also suitable for further uses such as immunohistochemical labeling and chromatographic blotting. Use it to quantify global hydroxymethylation, pull down 5-hmC fragments for sequencing or qPCR, or visualize the mark in tissue. Choose 25, 50 or 200 µg. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- Strong discrimination – Distinguishes 5-hmC from 5-mC and unmodified cytosine.
- Rabbit polyclonal – Recognizes 5-hydroxymethylcytosine in DNA samples.
- Validated applications – Tested in ELISA and immunoprecipitation enrichment.
- Additional uses – Suitable for immunohistochemistry and chromatographic blotting.
- Convenient sizes – Available in 25, 50 and 200 µg quantities.
Specifications
| Catalog Number | A4001-50 |
| Brand | Zymo Research |
| Description | Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl |
| Host / clonality | Rabbit polyclonal |
| Target | 5-hydroxymethylcytosine |
| Concentration | 1 µg/µl |
| Validated uses | ELISA, immunoprecipitation |
| Pack sizes | 25 µg; 50 µg; 200 µg |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Available Sizes & Components
- A4001-200 – Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl
- A4001-25 – Anti-5-Hydroxymethylcytosine Polyclonal Antibody, 1 µg/µl
Applications
- hMeDIP enrichment of 5-hmC DNA
- 5-hmC ELISA quantification
- Immunohistochemical labeling of 5-hmC
- Dot and chromatographic blotting
- Epigenetics research
