Product Description
LIGHT LABS is proud to offer the Accuris AccuFi™ High Fidelity Hot Start PCR Master Mix (Cat # PR1002-HFHS-100) — the newest and most advanced high fidelity PCR master mix in the Accuris Instruments lineup, delivering ultra-high fidelity DNA synthesis, antibody-mediated hot start specificity, rapid extension kinetics, and robust long-range amplification in a convenient pre-optimized 2X master mix format. Designed for researchers who demand both maximum sequence accuracy and maximum amplification performance, the AccuFi™ PCR Master Mix is the premier choice for molecular cloning, NGS library construction, site-directed mutagenesis, and any application where sequence integrity is critical.
The AccuFi™ formulation features a specially engineered high fidelity DNA polymerase possessing both 5′→3′ DNA polymerase activity and intrinsic 3′→5′ exonuclease proofreading activity, resulting in an error rate below the current limit of detection — significantly exceeding the accuracy of conventional high fidelity enzymes. The antibody-mediated hot start mechanism keeps the polymerase inactive at room temperature, completely eliminating non-specific amplification, primer-dimer formation, and background signal during reaction assembly. With a rapid extension rate of approximately 1 kb per 10 seconds, the AccuFi™ Master Mix supports fast cycling protocols without sacrificing fidelity or yield, and reliably amplifies long and challenging templates exceeding 15 kb — including GC-rich sequences, low-quality DNA, and partially impure samples. LIGHT LABS is your trusted source for Accuris Instruments PCR reagents.
KEY FEATURES
- Ultra-high fidelity — error rate below the detection limit, significantly exceeding conventional high fidelity PCR enzymes; ideal for cloning, mutagenesis, and NGS library construction where sequence integrity is critical
- Dual enzymatic activity: 5′→3′ DNA polymerase PLUS intrinsic 3′→5′ exonuclease proofreading for maximum accuracy
- Antibody-mediated hot start — polymerase completely inactive at room temperature, eliminating non-specific amplification and primer-dimer formation; convenient room temperature reaction assembly
- Fast extension kinetics — approximately 1 kb per 10 seconds enabling accelerated, fast cycling PCR protocols
- Long-range amplification — reliable performance on templates exceeding 15 kb
- Robust amplification across challenging templates — GC-rich sequences, low-quality DNA, and partially impure samples
- Optimized 2X master mix format — includes AccuFi™ polymerase, proprietary reaction buffer, MgCl₂, and ultra-pure dNTPs; simply add primers and template
- Reduces pipetting steps and improves throughput and reproducibility vs. component-based high fidelity PCR setups
- Highly efficient buffer system maintains high yield and specificity with minimal reaction optimization required
- Leaves a blunt end product — compatible with blunt-end cloning strategies
- 100-reaction format — ideal for routine high-fidelity PCR workflows
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | PR1002-HFHS-100 |
| Brand | Accuris Instruments / AccuFi™ |
| Product Name | AccuFi™ High Fidelity Hot Start PCR Master Mix |
| Concentration | 2X |
| Reaction Size | 25 µL reactions |
| Number of Reactions | 100 (at 25 µL) |
| Polymerase | AccuFi™ High Fidelity Hot Start DNA Polymerase |
| Fidelity | Ultra-high — error rate below detection limit |
| Hot Start Type | Antibody-mediated |
| Extension Rate | ~1 kb per 10 seconds |
| Long-Range Capability | Reliably amplifies templates >15 kb |
| Product End | Blunt end |
| Template Compatibility | Standard, GC-rich, low-quality, partially impure DNA |
| Components Included | 2X AccuFi™ Master Mix (AccuFi polymerase, proprietary buffer, MgCl₂, ultra-pure dNTPs) |
| Storage Temperature | -20°C |
APPLICATIONS
- Molecular cloning requiring maximum sequence fidelity (blunt-end cloning)
- Site-directed mutagenesis
- Next-generation sequencing (NGS) library construction and amplification
- Long-range PCR amplification (up to and beyond 15 kb)
- GC-rich and AT-rich template amplification
- Amplification of low-quality or partially impure DNA samples
- Genomics and whole genome amplification workflows
- High-throughput PCR requiring fast cycling with maximum fidelity
- Any PCR application where sequence integrity is paramount
ORDERING INFORMATION
| Cat # | Description | Concentration | Reactions |
|---|---|---|---|
| PR1002-HFHS-100 | Accuris AccuFi™ HF Hot Start PCR Master Mix | 2X | 100 x 25 µL |
| PR1002-HFHS-200 | Accuris AccuFi™ HF Hot Start PCR Master Mix | 2X | 200 x 25 µL |
| PR1002-HFHS-500 | Accuris AccuFi™ HF Hot Start PCR Master Mix | 2X | 500 x 25 µL |
| PR1001-HFHS-200 | Accuris High Fidelity Hot Start Master Mix | 2X | 200 x 25 µL |
| PR1001-HFHS-500 | Accuris High Fidelity Hot Start Master Mix | 2X | 500 x 25 µL |
| PR1001-HF-200 | Accuris High Fidelity Master Mix | 2X | 200 x 25 µL |
| PR1001-HF-500 | Accuris High Fidelity Master Mix | 2X | 500 x 25 µL |
| PR1000-HF-200 | Accuris High Fidelity DNA Polymerase | 2 U/µL | 200 U |
| PR1000-HF-1000 | Accuris High Fidelity DNA Polymerase | 2 U/µL | 1000 U |
