Product Description
LIGHT LABS is proud to offer the Accuris Fast Extraction PCR Kit (Cat # PR1400-FE-100) — a complete, all-in-one DNA extraction and PCR amplification kit that takes researchers from raw biological sample to amplified PCR product in less than 60 minutes, with only 8 minutes of actual extraction time. Designed for exceptional versatility across a wide range of sample types including animal tissue, plant material, saliva, and bacteria, the Fast Extraction PCR Kit eliminates the need for lengthy column-based DNA purification — dramatically reducing hands-on time, reagent costs, and the risk of sample loss or contamination during processing.
The kit features two proprietary components working in tandem: the exclusive single-tube Fast Extraction Lysis Solution that extracts genomic DNA in just 8 minutes at a single temperature step, and Accuris High Fidelity Hot Start Red Dye Master Mix — a 2X, pre-optimized formulation providing superior endpoint PCR performance with hot start specificity and a built-in non-reactive red dye for direct gel loading without a separate loading buffer step. The PCR-prepared genomic DNA can be used directly in endpoint PCR with the included master mix, or alternatively amplified via real-time qPCR using either SYBR Green or TaqMan probe chemistries with a compatible qPCR master mix (not included). LIGHT LABS is your trusted source for Accuris Instruments PCR reagents.
KEY FEATURES
- Complete extraction-to-amplification workflow in less than 60 minutes — from raw sample to PCR product
- Exclusive single-tube Fast Extraction Lysis Solution extracts genomic DNA in just 8 minutes
- No column purification required — eliminates lengthy extraction steps, reduces hands-on time and reagent costs
- Compatible with a wide range of sample types: animal tissue, plant material, saliva, and bacteria
- Includes Accuris High Fidelity Hot Start Red Dye Master Mix (2X) — provides hot start specificity to prevent non-specific amplification, with built-in non-reactive red dye for direct gel loading
- Red dye master mix enables direct gel loading without a separate loading buffer step — fewer pipetting steps and reduced contamination risk
- Dual-workflow versatility — extracted DNA compatible with endpoint PCR (using included master mix) OR real-time qPCR with SYBR Green or TaqMan probe chemistries (qPCR master mix not included)
- Hot start mechanism eliminates non-specific amplification and primer-dimer formation
- Simple, streamlined protocol optimized for routine genomic DNA extraction and PCR from difficult sample types
- Quality tested to ISO 9001:2015 standards
- 100-reaction kit — sufficient for routine genotyping and genomics workflows
SPECIFICATIONS
| Attribute | Detail |
|---|---|
| Catalog Number | PR1400-FE-100 |
| Brand | Accuris Instruments |
| Product Name | Fast Extraction PCR Kit |
| Number of Reactions | 100 (at 25 µL per reaction) |
| Extraction Time | ~8 minutes |
| Total Workflow Time | Less than 60 minutes |
| Sample Compatibility | Animal tissue, plant, saliva, bacteria |
| Master Mix Concentration | 2X |
| Hot Start Type | Yes — hot start formulation |
| Loading Dye | Non-reactive red dye included — enables direct gel loading |
| qPCR Compatibility | Yes — SYBR Green or TaqMan (qPCR master mix not included) |
| Kit Components | High Fidelity Hot Start Red Dye Master Mix (1.25 mL); Fast Extraction Lysis Solution (1.25 mL) |
| Storage Temperature | -20°C |
| Quality Standard | ISO 9001:2015 |
WORKFLOW OVERVIEW
| Step | Action | Time |
|---|---|---|
| 1 | Add Fast Extraction Lysis Solution to sample | 1 min |
| 2 | Incubate at extraction temperature | 8 min |
| 3 | Dilute lysate | 1 min |
| 4 | Set up PCR with included master mix | 5 min |
| 5 | PCR amplification | ~30–45 min |
| 6 | Direct gel loading (no loading buffer needed) | — |
| Total | Extraction + Amplification | < 60 min |
APPLICATIONS
- Rapid genomic DNA extraction and PCR amplification from animal tissue
- Plant genotyping and screening
- Bacterial colony PCR and genotyping
- Saliva-based DNA extraction and amplification
- Routine genotyping workflows requiring rapid sample-to-result turnaround
- Endpoint PCR screening and diagnostics
- Real-time qPCR with SYBR Green or TaqMan (using separate qPCR master mix)
- High-throughput genotyping workflows where speed and simplicity are critical
- Any workflow requiring rapid extraction and PCR from crude biological samples
ORDERING INFORMATION
| Cat # | Description | Reactions |
|---|---|---|
| PR1400-FE-100 | Fast Extraction PCR Kit | 100 x 25 µL |
| PR1400-FLS-100 | Fast Extraction PCR Kit (alternative format) | 100 x 25 µL |
| PR1002-HFHS-100 | AccuFi™ HF Hot Start PCR Master Mix | 100 x 25 µL |
| PR1002-HFHS-200 | AccuFi™ HF Hot Start PCR Master Mix | 200 x 25 µL |
| PR1001-HFHS-200 | High Fidelity Hot Start Master Mix | 200 x 25 µL |
| PR1001-HFHS-500 | High Fidelity Hot Start Master Mix | 500 x 25 µL |
| PR1001-HF-200 | High Fidelity Master Mix | 200 x 25 µL |
| PR1001-HF-500 | High Fidelity Master Mix | 500 x 25 µL |
