Product Description
The CELLTREAT® 229225B is a 25mL serological pipet individually wrapped in a paper/plastic wrapper and packed in bags — sterile, color-coded red, and manufactured to the ASTM Standard Specification for Serological Pipets E-934. Bi-directional (positive and negative) graduations are printed in bold, easy-to-read ink, and color-coded stripes and packaging enable quick volume identification at a glance. Each pipet features a premium material barrier plug for aerosol and contamination protection. Manufactured from USP Class VI medical grade virgin polystyrene — RNase-free and DNase-free. The 229225B is an improved longer-length 25mL serological pipet with extended -10mL graduations below zero, providing additional pipetting flexibility for blowout and overfill applications. Sold in cases of 200, packed in bags of 50.
ASTM E-934 Manufactured — Meets the Standard for Serological Pipets All CELLTREAT serological pipets are manufactured to the ASTM Standard Specification for Serological Pipets E-934 — the defining standard for dimensional accuracy, volumetric performance, and graduation requirements for serological pipets. Compliance with ASTM E-934 ensures consistent, reliable volume delivery that meets the expectations of quality-controlled cell culture labs, molecular biology environments, and regulated research settings requiring documented consumable qualification for GMP or GLP work.
25mL Format — The Essential Volume for Large-Scale Cell Culture The 25mL serological pipet is indispensable in high-volume cell culture labs — the go-to format for adding and aspirating media from T-75, T-150, and larger cell culture flasks, transferring large volumes of buffer and reagent, washing cell pellets in 50mL centrifuge tubes, and any liquid handling step requiring volumes between 10mL and 25mL. The 229225B is compatible with all major serological pipet controllers including the CELLTREAT ali-Q series, Corning Stripettor®, Integra VIAFLO, and all other commonly used controllers.
Extended -10mL Graduations — Improved Versatility The CELLTREAT 229225B features an improved longer length with extended -10mL graduations below zero — providing additional scale below the zero mark for blowout delivery, overfill applications, and pipetting techniques that require reading below the standard graduation range. This extended graduation range is a meaningful functional improvement over standard 25mL serological pipets that graduate only to zero, giving the 229225B added versatility across a broader range of pipetting protocols.
Bi-Directional Graduations — Positive and Negative Volume Delivery The 229225B features bi-directional (positive and negative) graduation markings printed in bold, easy-to-read ink — allowing the pipet to be read from either end of the scale. Positive graduations support standard delivery from full to empty, while negative graduations allow visual confirmation of volume remaining during use. The extended -10mL graduation markings provide additional reading range below zero for blowout and overfill techniques.
Premium Material Barrier Plug — Aerosol and Contamination Protection Each 229225B pipet includes a premium material barrier plug positioned to prevent liquid aerosols from being aspirated into the pipet controller during use — protecting the controller from contamination and preventing cross-contamination between samples. The plug material is selected for compatibility with aqueous media, buffers, sera, enzyme solutions, and all other common biological laboratory fluids.
Color-Coded Red — Instant 25mL Visual Identification The 229225B is color-coded red throughout — the pipet stripe, the wrapper, and the bag packaging all carry the red color code universally associated with the 25mL serological pipet format. In active cell culture labs where multiple pipet volumes are in simultaneous use, the red color coding allows instant visual identification of the correct volume without reading the label — reducing errors and streamlining workflow at the biosafety cabinet.
Individually Wrapped — Sterile Packaging for Single-Use Applications Each pipet is individually wrapped in a paper/plastic wrapper and packed in resealable bags of 50 — maintaining sterility until the moment of use while keeping the remaining pipets in the bag clean, organized, and accessible. The paper/plastic wrapper format is compatible with all standard biosafety cabinet and clean bench workflows and is the most widely used serological pipet packaging format in cell culture labs worldwide.
USP Class VI Polystyrene — RNase-Free and DNase-Free CELLTREAT serological pipets are manufactured from USP Class VI medical grade virgin polystyrene and are certified RNase-free and DNase-free — meeting the material purity standards required for molecular biology, RNA work, and sensitive cell culture applications. All pipets are supplied sterile by gamma irradiation.
Complete CELLTREAT® Individually Wrapped Serological Pipet Family:
| Cat. # | Volume | Color Code | Packaging | Qty/Case |
|---|---|---|---|---|
| 229001B | 1mL | Red | Indiv. wrapped, paper/plastic, bag | 500 |
| 229002B | 2mL | Yellow | Indiv. wrapped, paper/plastic, bag | 500 |
| 229005B | 5mL | Blue | Indiv. wrapped, paper/plastic, bag | 200 |
| 229205B | 5mL Extended | Blue | Indiv. wrapped, paper/plastic, bag | 200 |
| 229210B | 10mL | Orange | Indiv. wrapped, paper/plastic, bag | 200 |
| 229225B | 25mL | Red | Indiv. wrapped, paper/plastic, bag | 200 |
| 229230B | 50mL | Purple | Indiv. wrapped, paper/plastic, bag | 90 |
Key Features
- 25mL serological pipet — improved longer length with extended -10mL graduations
- Manufactured to ASTM Standard Specification E-934
- Individually wrapped in paper/plastic wrapper
- Packed in bags of 50 — 200 per case
- Bi-directional (positive and negative) graduations in bold easy-to-read ink
- Extended -10mL graduation scale below zero
- Premium material barrier plug — aerosol and contamination protection
- Color-coded red — universal 25mL identification
- USP Class VI medical grade virgin polystyrene
- RNase-free and DNase-free
- Sterile — gamma irradiation
- Compatible with all major serological pipet controllers
Supported Applications Large-volume cell culture media addition and aspiration from T-75, T-150, and larger flasks, bulk reagent preparation and transfer, cell pellet washing in 50mL tubes, serial dilutions of large volumes, microbiology assays, molecular biology liquid handling, serum and supplement addition in large volumes, and any laboratory application requiring a sterile 25mL individually wrapped serological pipet with extended graduation range.
Technical Specifications
| Specification | Value |
|---|---|
| SKU / Part Number | 229225B |
| Manufacturer | CELLTREAT Scientific Products |
| Volume | 25mL |
| Graduations | Bi-directional (positive and negative), extended to -10mL |
| Material | USP Class VI medical grade virgin polystyrene |
| Barrier Plug | Yes — premium material |
| Color Code | Red |
| Standard | ASTM E-934 |
| Sterility | Sterile — gamma irradiation |
| RNase-Free | Yes |
| DNase-Free | Yes |
| Packaging | Individually wrapped, paper/plastic |
| Pack Format | 50 per bag |
| Quantity per Case | 200 |