Product Description
Generating fully methylated DNA controls becomes straightforward when 5-methyl-dCTP is already blended into your nucleotide mix. This ready-to-use Zymo Research mix lets you build amplicons in which every cytosine carries a methyl group, giving you reliable reference material for testing bisulfite conversion, methylation-sensitive enzymes and other epigenetic assays without separate in vitro methylation steps.
This listing is for catalog number D1030: 5-Methylcytosine dNTP Mix (10 mM), 250 µl. The mix is supplied at 10 mM and contains 5-methyl-dCTP in place of standard dCTP alongside the other deoxynucleotides. Simply add it to your PCR setup with ZymoTaq, QuestTaq or another compatible DNA polymerase. The highly pure formulation supports clean amplification, and the product ships on dry ice so it should be stored frozen and thawed on ice before use. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- Pre-blended convenience – Methylated cytosine nucleotide is already combined with the other dNTPs for quick setup.
- Methylated amplicons – Produces PCR products carrying 5-methylcytosine for use as positive controls.
- High purity – Clean formulation supports consistent amplification results.
- Broad polymerase compatibility – Works with ZymoTaq, QuestTaq and other DNA polymerases.
- Dry ice shipping – Shipped frozen to protect nucleotide integrity.
Specifications
| Catalog Number | D1030 |
| Brand | Zymo Research |
| Description | 5-Methylcytosine dNTP Mix (10 mM), 250 µl |
| Concentration | 10 mM |
| Volume | 250 µl |
| Compatible polymerases | ZymoTaq, QuestTaq and other DNA polymerases |
| Shipping | Dry ice |
| Shipping Conditions | Ships on dry ice |
| Storage | -20°C |
Applications
- Methylated DNA control generation
- Bisulfite conversion validation
- Methylation-sensitive restriction enzyme testing
- Epigenetics assay development
