Product Description
Screening large yeast libraries goes faster when plasmid recovery runs in a plate. This Zymo Research kit brings the Zymoprep II chemistry to a 96-well format, letting you process many samples in parallel while recovering about five times more plasmid DNA than the Zymoprep I method and avoiding isopropanol precipitation.
This listing is for catalog number D2005: Zymoprep-96 Yeast Plasmid Miniprep, 2 x 96 Preps. Zymolyase is included in the first solution to digest fungal cell walls, followed by alkaline lysis, neutralization and binding to a 96-well purification plate. No glass beads or phenol are required. The kit supports S. cerevisiae, C. albicans, S. pombe and other susceptible fungi, and eluted DNA is suitable for E. coli transformation, PCR and Southern blot analysis. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- 96-well throughput – Processes many yeast samples in parallel for library and screening work.
- Higher yield – Recovers about five times more plasmid DNA than Zymoprep I.
- No precipitation – Plate-based purification removes the isopropanol step.
- No beads or phenol – Enzymatic cell wall digestion keeps the workflow simple and safer.
- Flexible inputs – Accepts colonies, plate patches or liquid cultures.
Specifications
| Catalog Number | D2005 |
| Brand | Zymo Research |
| Description | Zymoprep-96 Yeast Plasmid Miniprep, 2 x 96 Preps |
| Format | 96-well; 2 x 96, 4 x 96 or 8 x 96 preps |
| Relative yield | About 5-fold more plasmid DNA than Zymoprep I |
| Compatible organisms | S. cerevisiae, C. albicans, S. pombe and fungi susceptible to yeast lytic enzyme |
| Sample types | Colonies, plate patches, liquid cultures |
| Shipping Conditions | Ships at room temperature |
| Storage | -20°C |
| Note | [Includes E1004 x 2: Zymolyase (1 x 2000 Units Lyophilized) w/ Storage Buffer (500µl)] |
Available Sizes & Components
- D2006 – Zymoprep-96 Yeast Plasmid Miniprep, 4 x 96 Preps
- D2007 – Zymoprep-96 Yeast Plasmid Miniprep, 8 x 96 Preps
Applications
- Yeast two-hybrid library screening
- High-throughput plasmid rescue
- Shuttle vector recovery for E. coli transformation
- PCR and Southern blot analysis
