Product Description
Scaling methylation studies to hundreds of samples is far more practical when DNA purification can be skipped entirely. This 96-well bisulfite kit converts DNA straight from blood, tissue, and cells in plate format, offering the throughput large cohorts need with sensitivity down to 10 cells or 50 pg of DNA.
This listing is for catalog number D5023: EZ-96 DNA Methylation-Direct Kit (Deep-well), 2 x 96 Reactions. Samples are digested and converted directly, then desulphonated and purified on a 96-well spin plate so that converted DNA can be eluted for amplification. The kit comes in shallow-well and deep-well formats, each providing 2 x 96 reactions. Recovered DNA supports PCR followed by restriction digestion, sequencing, and microarray analysis. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- 96-well throughput – Processes full plates of samples in parallel.
- No prior extraction – Converts DNA directly from blood, tissue, and cells.
- High sensitivity – Supports amplification from as few as 10 cells or 50 pg DNA.
- Plate options – Available in shallow-well and deep-well formats.
- Complete conversion – Designed for reliable, thorough bisulfite conversion.
Specifications
| Catalog Number | D5023 |
| Brand | Zymo Research |
| Description | EZ-96 DNA Methylation-Direct Kit (Deep-well), 2 x 96 Reactions |
| Format | 96-well; shallow-well (D5022) or deep-well (D5023) |
| Reactions | 2 x 96 |
| Sample types | Blood, tissue, cells |
| Minimum input | 10 cells or 50 pg DNA |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Population-scale methylation studies
- High-throughput methylation-specific PCR
- Bisulfite sequencing
- Methylation microarrays
