Product Description
Convert a full plate of samples for methylation analysis in one run. This 96-well spin-plate kit brings streamlined bisulfite conversion to large cohort and screening studies, with deep-well and shallow-well options to suit your equipment. It is well suited to array studies. It also helps standardize conversion across large sample sets.
This listing is for catalog number D5004: EZ-96 DNA Methylation Kit (deep-well), 2 x 96 Reactions. Unmethylated cytosines are converted to uracil by sodium bisulfite in a three-step reaction, and in-column desulphonation removes the need for precipitations. The kit is designed to limit template degradation and DNA loss while achieving complete conversion. Converted DNA is ready for PCR, endonuclease digestion, sequencing and microarrays; the deep-well format (D5004) is recommended for Illumina Infinium MethylationEPIC BeadChips. Order it from LIGHT LABS and keep your workflow moving.
Key Features
- 96-well spin plate – Converts and cleans up full plates in parallel.
- Complete conversion – Designed to fully convert unmethylated cytosines.
- Minimal DNA loss – Limits template degradation during treatment and clean-up.
- No precipitations – In-column desulphonation.
- Two formats – Shallow-well and deep-well; deep-well recommended for EPIC arrays.
Specifications
| Catalog Number | D5004 |
| Brand | Zymo Research |
| Description | EZ-96 DNA Methylation Kit (deep-well), 2 x 96 Reactions |
| Format | 96-well spin plate (shallow-well or deep-well) |
| Chemistry | Sodium bisulfite conversion |
| Desulphonation | In-column |
| Recommended for | Illumina Infinium MethylationEPIC BeadChip (D5004) |
| Pack size | 2 x 96 reactions |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Methylation arrays
- Epigenome-wide association studies
- Bisulfite sequencing
- Methylation-specific PCR
- Epigenetic biomarker screening
