Product Description
Isolating intact nuclei is the gateway to clean chromatin, whether the end goal is immunoprecipitation or nucleosome analysis. This replacement buffer prepares cross-linked cell pellets for chromatin immunoprecipitation in the Zymo-Spin ChIP Kit, helping keep upstream sample processing consistent across experiments and batches. It is a simple way to restock a frequently used reagent.
This listing is for catalog number D5220-2-30: Nuclei Prep Buffer, 30 mL. Resuspend cross-linked cells in the buffer as directed in the Zymo-Spin ChIP protocol to release nuclei before chromatin shearing. It matches the kit-supplied component and is available in 30 ml and 50 ml bottles, so you can restock according to how many preparations you run. Store and handle it as described in the kit protocol. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Nuclei release – Prepares cross-linked cell pellets for ChIP.
- Kit-matched component – Same buffer supplied in the Zymo-Spin ChIP Kit.
- Two volumes – Offered in 30 ml and 50 ml bottles.
- Upstream consistency – Standardizes the first step of chromatin preparation.
Specifications
| Catalog Number | D5220-2-30 |
| Brand | Zymo Research |
| Description | Nuclei Prep Buffer, 30 mL |
| Volumes | 30 ml (D5220-2-30), 50 ml (D5220-2) |
| Sample type | Cross-linked cell pellets |
| For use with | Zymo-Spin ChIP Kit |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Available Sizes & Components
Applications
- Nuclei isolation for ChIP
- Zymo-Spin ChIP Kit replenishment
- Chromatin preparation
