Product Description
Reproducible micrococcal nuclease digestion depends on giving the enzyme the right reaction conditions every time. This replacement buffer is designed for MNase digestion of DNA and RNA in the EZ Nucleosomal DNA Prep Kit, supporting complete enzyme activity so nucleosomal fragments are released consistently. Replacing it separately extends the life of an opened kit.
This listing is for catalog number D5220-3: MN Digestion Buffer, 50 mL. Resuspend isolated nuclei in the buffer before adding Micrococcal Nuclease, following the EZ Nucleosomal DNA Prep protocol. It is the same buffer included in the kit (D5220), is sold separately in a 50 ml bottle, and is stored at room temperature. Pair it with 5X MN Stop Buffer to end the reaction. LIGHT LABS ships genuine Zymo Research products with knowledgeable support from our Englewood, Colorado team.
Key Features
- Optimal activity – Provides conditions for complete micrococcal nuclease activity.
- Kit-matched component – Same buffer supplied in the EZ Nucleosomal DNA Prep Kit.
- 50 ml bottle – Supplied as a standalone 50 ml volume.
- Room temperature storage – No refrigeration needed.
Specifications
| Catalog Number | D5220-3 |
| Brand | Zymo Research |
| Description | MN Digestion Buffer, 50 mL |
| Volume | 50 ml |
| Storage | Room temperature |
| For use with | EZ Nucleosomal DNA Prep Kit (D5220) |
| Shipping Conditions | Ships at room temperature |
| Storage | Room Temperature |
Applications
- MNase digestion of chromatin
- Nucleosomal DNA preparation
- EZ Nucleosomal DNA Prep Kit replenishment
