Product Description
Global methylation levels can be measured in an afternoon without sequencing or specialized instruments. This ELISA quantifies 5-methylcytosine in DNA samples in under three hours, using a sensitive and specific monoclonal antibody and a simple plate workflow that scales easily to large numbers of samples.
This listing is for catalog number D5326: 5-mC DNA ELISA Kit, 2 x 96 Preps. Sample DNA is coated onto the plate and then detected with an anti-5-methylcytosine monoclonal antibody and an HRP-based secondary detection system. Included controls are used to build a standard curve from which percent 5-mC is calculated. The assay accepts vertebrate, plant, and microbial DNA, as well as methylated PCR products and fragmented DNA. Kits contain 96 or 2 x 96 preps. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Fast assay – Quantifies 5-mC in less than 3 hours.
- Specific antibody – Uses a sensitive and specific anti-5-methylcytosine monoclonal antibody.
- Broad sample range – Works with vertebrate, plant, microbial, amplified, and fragmented DNA.
- Standard curve quantitation – Included controls allow calculation of percent 5-mC.
- High-throughput format – Plate workflow suits large sample sets.
Specifications
| Catalog Number | D5326 |
| Brand | Zymo Research |
| Description | 5-mC DNA ELISA Kit, 2 x 96 Preps |
| Assay time | Less than 3 hours |
| Antibody | Anti-5-Methylcytosine monoclonal |
| Compatible DNA | Vertebrate, plant, microbial, methylated PCR amplicons, fragmented DNA |
| Quantification | Standard curve from included controls |
| Kit sizes | 96 (D5325), 2 x 96 (D5326) preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- Global DNA methylation quantification
- Drug and inhibitor screening
- Plant and microbial methylation studies
- Methylated amplicon analysis
