Product Description
Screening many samples for 5-hydroxymethylcytosine is far easier with a plate assay than with sequencing-based methods. This ELISA offers sensitive, specific detection of 5-hmC across a broad range of sample types, and its streamlined workflow makes it practical for comparing large sample sets. Results are reported as percent 5-hmC.
This listing is for catalog number D5426: Quest 5-hmC DNA ELISA Kit, 2 x 96 Preps. The kit accepts intact genomic DNA from vertebrates, plants, and microbes, as well as enzyme-digested or mechanically sheared fragments. A calibrated Control DNA Set is used to generate a standard curve from which percent 5-hmC is calculated. Components include coating buffer, ELISA buffer, an anti-DNA HRP antibody, and HRP developer. Choose 96 or 2 x 96 preps. Add it to your LIGHT LABS order and keep your bench stocked.
Key Features
- Sensitive and specific – Detects 5-hmC accurately across varied samples.
- Broad input range – Accepts intact vertebrate, plant, and microbial DNA or fragmented DNA.
- Calibrated controls – Control DNA Set enables percent 5-hmC quantitation.
- Screening friendly – Streamlined plate workflow suits large sample numbers.
- Two kit sizes – Offered as 96 or 2 x 96 preps.
Specifications
| Catalog Number | D5426 |
| Brand | Zymo Research |
| Description | Quest 5-hmC DNA ELISA Kit, 2 x 96 Preps |
| Compatible DNA | Intact vertebrate, plant, microbial gDNA; enzyme-digested or sheared fragments |
| Quantification | Standard curve from calibrated Control DNA Set |
| Kit sizes | 96 (D5425), 2 x 96 (D5426) preps |
| Shipping Conditions | Ships on dry ice |
| Storage | -80C/4C |
Available Sizes & Components
Applications
- Global 5-hmC quantification
- Neuroscience and stem cell epigenetics
- Screening of large sample sets
- Comparison of tissue hydroxymethylation
