Product Description
Signal generation in a plate-based hydroxymethylation assay depends on a well-matched enzyme-linked antibody. This 100X anti-DNA HRP antibody is the replacement detection reagent for the Quest 5-hmC DNA ELISA Kit, supporting sensitive detection and quantification of DNA hydroxymethylation in your samples. Replacing it separately helps keep assay sensitivity consistent across experiments.
This listing is for catalog number D5425-3-200: Anti-DNA HRP Antibody (100X), 200 µl. Dilute the 100X stock as directed in the Quest 5-hmC DNA ELISA protocol, apply it to the plate at the detection step, and develop the signal with HRP Developer. It is the same antibody supplied in the complete kit and is available in 100 µl and 200 µl volumes. LIGHT LABS stocks the Zymo Research line, so kits, buffers and refills are always within reach.
Key Features
- HRP conjugated – Enables colorimetric detection with HRP Developer.
- 100X concentrate – Diluted to working strength before use.
- Kit-matched component – Same antibody supplied in the Quest 5-hmC DNA ELISA Kit.
- Two volumes – Available in 100 µl and 200 µl sizes.
Specifications
| Catalog Number | D5425-3-200 |
| Brand | Zymo Research |
| Description | Anti-DNA HRP Antibody (100X), 200 µl |
| Concentration | 100X |
| Volumes | 100 µl (D5425-3-100), 200 µl (D5425-3-200) |
| For use with | Quest 5-hmC DNA ELISA Kit |
| Shipping Conditions | Ships on dry ice |
| Storage | -80°C |
Available Sizes & Components
Applications
- 5-hmC ELISA detection
- DNA hydroxymethylation quantification
- Quest 5-hmC ELISA Kit replenishment
